刀豆蛋白A 来源于洋刀豆 (刀豆)
Concanavalin A from Canavalia ensiformis (Jack bean)
货号:MC2010-25mg
规格:25mg
价格:250.00元
Type IV, lyophilized powder
别名: 伴刀豆球蛋白A刀豆素 A
· CAS号 11028-71-0
type :
Type IV
form :
lyophilized powder
potency :
<64 μg per mL agglutination activity
impurities :
Carbohydrate, essentially free
salt, essentially free
storage temp. :
−20°C
Concanavalin A (con A) is a plant lectin refined from jack beans.It is a tetrameric protein, which has four identical 28 kDa subunits.Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.]
Concanavalin A from Canavalia ensiformis (Jack bean) has been used: • as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs) • for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis • for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay • for the stimulation of spleen in mitogen assays
刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。
通过特定亲和层析法进行高度纯化
据报道,凝集活性以μg/ml表示,并且在Ca2+ 和Mn2+ 浓度为1 mg/ml、pH 6.8的磷酸缓冲盐溶液中进行连续稀释测定。该活性是在25°C下孵育1小时的2%人红细胞悬浮液可以凝集的最低浓度。
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