RIN-5F大鼠胰腺组织/胰岛细胞瘤
Organism Rattus norvegicus, rat Tissue pancreas/islet cell tumor Cell Type beta cell Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender male Strain NEDH Applications These cells offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin. Storage Conditions liquid nitrogen vapor phase
传代方法： 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。 （一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。 (二)如果细胞已长满，即可进行传代培养。具体步骤如下： 1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。 2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。 3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 。 注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造 成生长不好。
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