A9 小鼠皮下结缔组织细胞
ATCC® Number: CCL-1.4™ Designations: A9 (APRT and HPRT negative derivative of Strain L) Depositors: TR Chen Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Mus musculus (mouse) Morphology: fibroblast
A9 小鼠皮下结缔组织细胞 Source: Strain: C3H/An Tissue: subcutaneous connective tissue; areolar and adipose Disease: normal Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Tumorigenic: Yes Antigen Expression: H-2k Cytogenetic Analysis: modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent. Age: 100 days Gender: male Comments: The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells. The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT). The cells are resistant to 8-azaguanine (0.003 mg/ml) and 6-thioguanine (0.1 mM). Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10% A9 小鼠皮下结缔组织细胞 Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove old medium, add fresh 0.25% trypsin, EDTA (0.03%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Related Products: parental cell line:ATCC CCL-1 References: 21409: Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106 21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673 27669: Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 A9 小鼠皮下结缔组织细胞
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