CRL-2279 MS1 小鼠胰岛内皮细胞 ATCC® Number: CRL-2279™ Designations: MS1 (MILE SVEN 1) Depositors: JL Arbiser Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ] Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Mus musculus (mouse) Morphology: endothelial
Source: Organ: pancreas Strain: C57BL/6 Tissue: islet of Langerhans; endothelium Cell Type: SV40 transformed Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels) Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Receptors: vascular endothelial growth factor (VEGF), expressed Comments: MS1 is a pancreatic islet endothelial cell line established in 1994. Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418. Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL. The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor. The cells are useful for the study of signal transduction of angigenic factors. It is the only line known that can give rise to benign hemangiomas. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347 51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370
沪公网安备 31010902002429号
