CRL-1949 Psi2 DAP 小鼠胚胎成纤维细胞 ATCC® Number: CRL-1949™ Designations: Psi2 DAP Depositors: CL Cepko Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Mus musculus (mouse) Morphology: fibroblast
Source: Strain: NIH/Swiss Organ: embryo Disease: normal Cellular Products: a human placental alkaline phosphatase transducing vector Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Reverse Transcript: positive Age: embryo Comments: This line produces a vector that can infect mouse cells. Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene. The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP). Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo. The cells may produce helper virus. To assay for helper virus, and for other retrovirus techniques, see Cepko, C.L. Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds., Humana Press, Clifton, NJ, 1989. Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10% Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks. References: 22598: Fields-Berry SC, et al. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA 89: 693-697, 1992. PubMed: 1731342
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