产品 资讯
请输入产品名称
噪声分析仪 纺织检测仪器 Toc分析仪 PT-303红外测温仪 转矩测试仪 继电保护试验仪 定氮仪
首页 产品 专题 品牌 资料 展会 成功案例 网上展会
词多 效果好 就选易搜宝！
首页 >>> 产品目录 >>> ATCC细胞、细胞株、肿瘤细胞 >>> 细胞系_小鼠正常细胞系目录
仪表展览网 >>> 展馆展区 >>> CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞 > CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞

产品资料

CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞

CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞
  • 如果您对该产品感兴趣的话，可以
  • 产品名称：CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞
  • 产品型号：CRL-1927
  • 产品展商：美国标准生物品收藏中心（ATCC）
  • 产品文档：无相关文档
简单介绍
CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
产品描述
CRL-1927 SV40 MES 13 小鼠肾小球系膜细胞
ATCC® Number: CRL-1927™    Price: $355.00
Designations: SV40 MES 13
Depositors:  LJ Striker
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: myofibroblast-like
Source: Organ: kidney
Tissue: glomerulus
Cell Type: mesangial cell;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Age: 7 to 10 weeks
Comments: The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1 X 10(-6) M angiotensin II. The cells form colonies in soft agar, and are positive for SV40 large T antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is 3:1 mixture of ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002 and Ham's F12 medium with 14 mM HEPES.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 26 hrs
Related Products: recommended serum:ATCC 30-2020
References: 22754: MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539
51029: Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 10564237
51030: Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int. 57: 2308-2318, 2000. PubMed: 10844601
61211: Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-binding epidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucose metabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activation and induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 11782486
61224: Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol. Histopathol. 15: 403-414, 2000. PubMed: 10809358
61225: Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 12110510
90657: Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed: 15070811
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
  • 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
  • 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报
产品留言
标题
内容
联系人
联系电话
电子邮件
公司名称
联系地址
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

沪公网安备 31010902002429号