活性氧(ROS)自由基可以直接攻击生物大分子DNA诱发DNA氧化损伤。在各种氧化损伤中，鸟苷是DNA核碱基中*易被氧化的碱基，羟基自由基和超氧阴离子会直接攻击DNA分子中鸟嘌呤碱基的第8位碳原子，从而生成氧化性加合物8-OhdG（8-羟基脱氧鸟 苷）。
8-OHdG现已被广泛地应用在实验研究和临床诊断中，参与氧化应激与病变的指标检测与机理分析，对心血管疾bing、糖尿病、神经退行性疾bing（帕金森病，阿尔茨海默氏症）、以及癌症的临床诊疗都具有极大的应用价值。目前市场上主流使用的是竞争ELISA方法，因为它们不够精que，也不便于应用于细胞/组织的8-OHdG检测，更不能直接检测从细胞或组织分离的完整DNA。
为此，艾美捷科技作为专业的生命科学领域解决方案供应商，为您推荐全球*新的8-OHdG直接定量试剂盒。其检测准确度与HPLC或LC-MS分析高度相关和接近。
*8-OHdG试剂盒可直接检测提取好的DNA样品
8-羟基脱氧鸟苷试剂盒原理
DNA包被特殊处理带有高DNA亲和性的微孔板上，8-OHdG通过捕获抗体和检测抗体来检测.通过酶标仪可以检测结果.大量得8-OHdG与光密度值成比例.
<8-OHdG试剂盒实验流程，标准曲线，以及与液相质谱分析对比>
8-OHdG试剂盒优势：
1、比色实验简单易用，方便快速，整个试验完成需要3小时45分钟；
2、高灵敏度，检测8-OHdG极限低至2pg；
3、高特异性，只能检测8-OHdG，不能检测8-OHdG类似物；
4、利用完整DNA直接检测8-OHdG，能消除高分子量化合物的影响，例如：糖类和蛋白质等能竞争8-OHdG；
5、检测结果精que，近似接近于HPLC和LC-MS；
6、高度方便直接使用来自细胞，组织中的DNA，不需要DNA消化或者水解；
7、包含通用的阳性对照和阴性对照，能适用于定量任何种系8-OHdG；
8、本试剂盒实验结果更精que，灵敏，可靠，可靠并能显著降低背景信号.
《8-OHdG试剂盒已发表文章》
Smolensky D et. al. (May 2018). High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis. J Med Food.
McLennan D et. al. (April 2018). Telomere elongation during early development is independent of environmental temperatures in Atlantic salmon. J Exp Biol.
Esplugas R et. al. (February 2018). Renal and hepatic effects following neonatal exposure to low doses of Bisphenol-A and <sup>137</sup>Cs. Food Chem Toxicol. 114:270-277.
Ceylan D et. al. (December 2017). DNA redox modulations and global DNA methylation in Bipolar Disorder: Effects of sex, smoking and illness state Psychiatry Research.
Haque S et. al. (November 2017). Monocyte-derived exosomes upon exposure to cigarette smoke condensate alter their characteristics and show protective effect against cytotoxicity and HIV-1 replication. Sci Rep. 7(1):16120. Tatsuki Kunoh et. al. (October 2017). Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation ACS Sustainable Chemistry & Engineering.
Zhenqiang You et. al. (September 2017). Modulatory Effect of Fermented Papaya Extracts on Mammary Gland Hyperplasia Induced by Estrogen and Progestin in Female Rats Oxidative Medicine and Cellular Longevity. 2017
Hara K et. al. (September 2017). Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells. PLoS One. 12(9):e0184332.
Afshari P et. al. (September 2017). Reduced Slc1a1 expression is associated with neuroinflammation and impaired sensorimotor gating and cognitive performance in mice: Implications for schizophrenia. PLoS One. 12(9):e0183854.
Tvrda et. al.(July 2017). Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservationVeterinarni Medicina. 62(8):429–436.
Bellés M et. al. (March 2017). Environmental exposure to low-doses of ionizing radiation. Effects on early nephrotoxicity in mice. Environ Res. 156:291-296.
Noguera JC et. al. (March 2017). Interacting effects of early dietary conditions and reproductive effort on the oxidative costs of reproduction. PeerJ. 5:e3094.
Ng CT et. al. (February 2017). Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and <i>Drosophila melanogaster</i>. Int J Nanomedicine. 12:1621-1637.
Tasaki E et. al. (January 2017). An Efficient Antioxidant System in a Long-Lived Termite Queen. PLoS One. 12(1):e0167412.
Daniel Nettle et. al. (December 2016). Early-life adversity accelerates cellular ageing and affects ***** inflammation: Experimental evidence from the European starling Scientific Reports. Dec. 2016
Szmidt M et. al. (July 2016). Toxicity of different forms of graphene in a chicken embryo model. Environ Sci Pollut Res Int.
Adamczyk J et. al. (June 2016). Copy number variations of genes involved in stress responses reflect the redox state and DNA damage in brewing yeasts. Cell Stress Chaperones.
Yost AD et. al. (October 2015). Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage. PLoS One. 10(10):e0139903.
Deregowska A et. al. (October 2015). Genome-wide array-CGH analysis reveals YRF1 gene copy number variation that modulates genetic stability in distillery yeasts. Oncotarget. 6(31):30650-63.
Philbrook NA et. al. (October 2015). Benzoquinone toxicity is not prevented by sulforaphane in CD-1 mouse fetal liver cells. J Appl Toxicol.
Shimizu R et. al. (April 2015). Cholangiocyte senescence caused by lysophosphatidylcholine as a potential implication in carcinogenesis. J Hepatobiliary Pancreat Sci.
Nocchi L et. al. (June 2014). Induction of oxidative stress causes functional alterations in mouse urothelium via a TRPM8-mediated mechanism: implications for aging. Aging Cell. 13(3):540-50.
Di Nunzio M et. al. (November 2013). Counteraction of oxidative damage by pomegranate juice: influence of the cultivar. J Sci Food Agric. 93(14):3565-73.
Sobinoff AP et. al. (September 2013). Scrambled and fried: cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress. Toxicol Appl Pharmacol. 271(2):156-67.
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