试虫和解剖
气候箱(培养试虫)
实体解剖镜和冷光源 (Leica)
任氏液 (inmM: 150 NaCl, 3 CaCl2, 3 KCl, 25 Sucrose, and 10 N-tris(hydroxymethyl)-methyl-2-amino-ethanesulfonic acid, pH 6.9)
弹簧剪刀(Fine ScienceTools)
精细镊子(Fine ScienceTools)
解剖针(Fine ScienceTools)
石蜡(固定试虫)(KERRcorporation)
细胞内记录和染色
荧光染料
Dextran,tetramethylrhodamine and biotin 3000 MW, lysine fixable(Micro-ruby), Invitrogen,
Lucifer Yellow(Invitrogen)
AxoClamp 2B (current andVoltage clamp) Axon Instruments,
防震工作台(TMC TechnicalManufacturing Corporation)
防静电网
玻璃毛细管Glass capillaries(Borosilicate glass capillaries, Hilgenberg GmbH, Germany; OD: 1mm, ID: 0.75 mm)
KAc (E. Merck)
LiCl (Fluka)
扬声器
微动仪 (Leica)
示波器
玻璃电极拉制仪 (Sutter InstrumentCo.)
气味刺激系统
刺激气味化合物
Z9-16:ALcis-9-hexadecenal
Z11-16:ALcis-11-hexadecenal
Z9-14:ALcis-9-tetradecenal
Z9-16:OHcis-9-hexadecenol
Z11-16:OHcis-11-hexadecenol
Z11-16:ACcis-11-hexadecenyl acetate
Ylang-Ylang (Plantoil,Dragoco)
**染色标记
甲醛(Merck)和多聚甲醛 (Merck)(固定液)
水杨酸甲酯(样本透明)
Permount
Xylene
琼脂糖(Sigma)(样本包埋)
清蛋白 (Fluka) (样本包埋)
白明胶 (Sigma) (样本包埋)
磷酸缓冲液 (PBS; in mM: 684NaCl, 13 KCl, 50.7 Na2HPO4, 5KH2PO4, pH 7.4)
Triton X-100(Sigma)
振动切片机 (Leica)
Alexa fluor R 647 goatanti-rabbit (Invitrogen)
Alexa fluor R 488 goatanti-mouse (Invitrogen)
ChAT 4B1 mouse monoclonalantibody (Developmental Studies Hybridoma Bank)
GABA antiserum(Sigma)
Locustatachykinin IIantiserum
Normal goat serum(Sigma)
Rabbit anti-serotonin(5-HT)(ImmunoStar)
Streptavidin-Cy3 (JacksonImmunoresearch)
SYNORF1 anti-synapsin Imouse monoclonal antibody (Developmental Studies HybridomaBank)
振荡器和摇床
移液器(Eppendorf)
pH 计
成像
Laser scanning confocalmicroscope (LSM 510, META Zeiss, Jena, Germany)
图像处理和数据分析
Adobe Photoshop CS2
Adobe Illustrator CS3
Amira (VisageImaging)
Spike2 6.02 (CED)
SPSS