EBERS vascular culture chamber (patent pending) is designed to grow vascular tissue on cylindrical-shaped scaffolds accounting for internal and external culture media flows. Furthermore, special effort has been spent in developing a simple system able to host a wide range of scaffold sizes, both in diameter and in length.
The most relevant parts of the vascular chamber and its working configuration are shown below.ch
Forming parts
The chamber is composed two parts forming internal and external flow circuits, respectively. The gap in the internal circuit is filled with vessel scaffold, allowing pumping fluid media inside the scaffold lumen.
The external circuit aims to circulate culture media over the external surface of the scaffold so nutrients are delivered to the outer surface.
Adaptable working length
In order to fit different scaffolds sizes, the working length in between the tube edges of the inner circuit may be adjusted, always guaranteeing no fluid leakage.
Since the scaffold diameter may be also vary depending of the kind of vessel, we can modify the dimensions of the tubing in order to customize the chamber for your particular application.
Working configuration
Once the chamber is locked, the system is ready to be connected to the culture circuit. The number of tube docks may be variable depending on the particular needs of the experiment.
Although the standard configuration it has four input/output ports, the chamber may be modified in order to adapt the number of ports to the demands of your experimental set-up.
After the scaffold is fixed closing the internal circuit and the chamber is locked, silicone tubing is connected to the ports to create two independent circuits.
Flow conditions may be independently applied to each of the circuits by using, e.g., the EBERS Master Unit.
Visit the technical specifications page for further information on the EBERS vascular chambers.