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  • 入驻时间: 2014-06-10
  • 联系人:业务代表
  • 电话:400-968-7988
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  • Email:fuwu@bioleaf.com
产品详情
  • 产品名称:SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml

  • 产品型号:上等胎牛血清http://www.shijichina.com/gibco/
  • 产品厂商:其它品牌
  • 产品价格:0
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简单介绍:
400-968-7988 SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中 上等胎牛血清http://www.shijichina.com/gibco/ 主营产品与服务:细胞株和菌株、胎牛血清、标准品和对照品、生化试剂、ELISA试剂盒、抗体和抗原、细胞因子、技术服务、实验耗材和消耗品、仪器设备SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中
详情介绍:

400-968-7988

GIBCO:A2720801  Fetal Bovine Serum, exosome-depleted 500ml
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml

SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中

上等胎牛血清http://www.shijichina.com/gibco/


SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中
上等胎牛血清http://www.shijichina.com/gibco/

主营产品与服务:细胞株和菌株、胎牛血清、标准品和对照品、生化试剂、ELISA试剂盒、抗体和抗原、细胞因子、技术服务、实验耗材和消耗品、仪器设备SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中

黑胶虫究竟是一种生物还是非生物,本身就存在争议。有人认为是从血清中来的一种原虫,也有人认为是细菌,或是真菌,也有人认为是血清中的蛋白的结晶。“黑胶虫”可寄生于动物细胞,也可以生存于培养基中,依靠细胞和培养基中的营养为生,并随细胞传代而传代。“黑胶虫”与细胞竞争性生长,开始时对细胞并没有什么影响,但当黑胶虫的数量多到一定程度的时候, 细胞生长就会受到影响直至死亡,严重影响了科研活动的开展和进行。

SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中
上等胎牛血清http://www.shijichina.com/gibco/

不管对于黑胶虫的争论如何,但有几个是目前大家公认的:
① 与细胞竞争性生长,对细胞生长有不利影响。
② “黑胶虫”会增殖,增殖多时,视野下一大片都为“黑胶虫”。
③ “黑胶虫”与血清有关,与血清质量有很大关系。

SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中
上等胎牛血清http://www.shijichina.com/gibco/
2、目前对“黑胶虫”的定论:有2种解释:
**,黑胶虫为生物。
它是小于细菌的生物,直径小于0.1μm,大多国外血清中多见,可能是国外牛血清中含有的某种原虫。只要它是生物,那么在培养基中一定会对细胞有影响。
**,黑胶虫不是生物。
国内的血清中,通常灭活之后都会有絮状沉淀出现,而黑胶虫出现时,所使用的血清被反复冻融过多次。所以推测,所谓的“黑胶虫”其实就是血清中的某些蛋白成分的物质,经过灭活之后丧失活性,在培养基中,镜检见到的运动其实是这些物质在溶液中做“布朗运动”。随着细胞消耗培养基,这些物质越来越多,*后细胞所用的营养消耗完,细胞容易死亡。


【黑胶虫大家的处理】


SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml 胎牛血清优惠中
上等胎牛血清http://www.shijichina.com/gibco/
1.换好一点的血清(我觉着和血清的关系不大)。
2.如果是贴壁细胞的话,可用无菌的PBS洗几次,可一定程度上缓解。若是悬浮的话,就不太好处理拉,可以向其中少加一点滋养细胞(从小鼠腹腔内取的巨噬细胞,这种细胞不分裂,过一阵就死掉了,做抗体杂交瘤融合的同志肯定知道)。加滋养细胞对有黑胶虫的刚复苏的细胞很有效!还有就是实验环境要注意好,保持细胞间整个环境的洁净度。
3.换用进口的一次性塑料培养瓶。
4.建议清理无菌室所有物品,重新灭菌,用KMnO4熏蒸,按**使用无菌室做,细胞重新复苏,。
5. 在换液前先加入生理盐水并轻轻拍打,冲洗干净后再加入培养液,坚持天天洗,传代时加生理盐水再离心一次,接种密度稍大一些,一段时间后,虫子就会大大减少,对细胞的生长也不会有大的影响。
6.有材料称minocycline具有一定的作用,可以和换液结合起来使用。
7.可以用专门的杀黑胶虫培养基。

SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco血清产品中文名称 货号 英文名称 规格 供应商
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco One Shot? 胎牛血清,优级 16000-077/16000-085/26140-129 One Shot? Fetal Bovine Serum, Certified 50ml/40*50ml/40*50ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco Sf-900 II SFM (1X), liquid 10902-096 Sf-900 II SFM (1X), liquid 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco Sf-900 Medium (1.3X), liquid 10967-032 Sf-900 Medium (1.3X), liquid 100ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco Sf-900? III SFM (1X),liquid 12658-019 Sf-900? III SFM (1X), liquid 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco羔羊血清 16070-096 Lamb Serum 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco供体牛血清 16030-074 Donor Bovine Serum 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco供体牛血清,加铁 10371-029 Donor Bovine Serum, with Iron 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco鸡血清 16110-082 Chicken Serum 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco马血清 16050-130/16050-122/16050-114 Horse Serum 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco马血清,热灭活 26050-070/26050-088 Horse Serum, Heat-Inactivated 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco牛血清 16170-086/16170-078/16170-060 Bovine Serum 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco牛血清,热灭活 26170-035/26170-043 Bovine Serum, Heat-Inactivated 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco山羊血清 16210-064/16210-072 Goat Serum 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,ES细胞,优级,FBS(US) 16141-061 Fetal Bovine Serum, ES Cell-Qualified FBS 100ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,ES细胞为优级的胎牛血清(美国) 16141-079 Fetal Bovine Serum, ES Cell-Qualified FBS 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,ES细胞优级 10439-024 Fetal Bovine Serum, ES Cell-Qualified 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,ES细胞优级(美国农业部批准) 10439-016 Fetal Bovine Serum, ES Cell-Qualified (USDA Approved) 100ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,MSC优级(美国农业部批准) 12662-029 Fetal Bovine Serum, MSC-Qualified (USDA Approved) 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,MS细胞优级,FBS 12763-025 Fetal Bovine Serum, MS Cell Quallified FBS 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,超低IgG 16250-086/16250-078 Fetal Bovine Serum, Ultra-Low IgG 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,特优级 16000-036/16000-044/16000-069 Fetal Bovine Serum,Certified 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,特优级,热灭活 10082-139/10082-147 Fetal Bovine Serum, Qualified,Heat-Inactivated 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,透析(美国) 26400-036/26400-044 Fetal Bovine Serum, Dialyzed(US) 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级 10099-133/10099-141/10099-158 Fetal Bovine Serum, Qualified 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级 10437-010/10437-028/10437-036 Fetal Bovine Serum, Qualified 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级 26140-087/26140-079/26140-095 Fetal Bovine Serum, Qualified 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级,热灭活 10100-139/10100-147/10100-154 Fetal Bovine Serum, Qualified,Heat-Inactivated 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级,热灭活 10438-018/10438-026/10438-034 Fetal Bovine Serum, Qualified,Heat-Inactivated 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级,热灭活 16140-063/16140-071/16140-089 Fetal Bovine Serum, Qualified,Heat-Inactivated 100ml/500ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级-MSC 12662-011 Fetal Bovine Serum, MSC-Qualified 100ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco胎牛血清,优级One Shot? 10437-077/10437-085 Fetal Bovine Serum, Qualified One Shot? 50ml/40*50ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco兔血清 16120-099/16120-107 Rabbit Serum 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco新生牛血清 16010-167/16010-142 Newborn Calf Serum 100ml/1000ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco新生牛血清,优级 16010-159 Newborn Bovine Serum,Qualified 500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco新生小牛血清,热灭活 26010-066/26010-074 Newborn Calf Serum, Heat-Inactivated 100ml/500ml GIBCO
SBI: EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250ml Gibco猪血清 26250-084 Porcine Serum 500ml GIBCO
无外泌体血清品牌 无外泌体血清货号 无外泌体血清品名 无外泌体血清规格
GIBCO A2720801
Fetal Bovine Serum, exosome-depleted
500ml
SBI EXO-FBS-250A-1
Exosome-depleted FBS Media Supplement
250ml

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