电融合电穿孔系统

ECM® 2001+是ECM2001的升级产品，属于一台多功能的细胞电融合和电穿孔仪器。ECM® 2001+能够快速高效制备杂交瘤细胞和进行核移植操作。独特的交流正弦波使细胞在双向电泳作用下排列到一起，然后在微秒级时间内转换成直流方波使其融合，融合后又一个短暂的交流脉冲稳定杂合细胞的融合状态，大大提高了细胞融合的效率。相对于ECM2001，交流频率可调节范围更大，使电融合效率更高。

ECM® 2001单独发生直流方波脉冲，可作为电穿孔系统，结合多种专业电极，适用于各种要求的电穿孔实验，广泛用于离体和活体电穿孔。

特点：

·         AC 频率可调节  0.2 – 2.0 MHz

·         直流方波脉冲可单独作为电穿孔系统

·         电压调节范围在5 V 到 3000 V

·         电压调节分辨率更高

·         一个程序可设置多个AC步骤

·         低电阻下也能操作

·         7寸触摸屏操作，界面直观方便

多功能
兼有细胞电融合和电穿孔功能，满足从简单转染到细胞融合一系列实验操作。
灵活性
拥有较宽的电压和脉冲时间范围，可根据实验方案调整所有脉冲参数，可在8Ω低阻抗下操作；融合过程在显微镜下可视操作，提高细胞融合精度。
快速高效 
通过交流非正弦波和直流方波电脉冲的交替作用，可以简单而快速地完成细胞排列、融合、融合后处理全过程，仅需几秒钟。
**性能
电弧淬灭功能，降低电弧引起的损害；短路保护，避免脉冲发生器遇到短路时被损坏。
兼容性
除35mm培养皿电极之外的其他所有BTX电极， 可配2ml和9ml电融合池，提高效率。Enhancer3000监测系统，脚控开关
监控仪选配
Enhancer 3000可以允许用户监控和记录主要的电流参数，利用选配的通讯模块，可以把数据下载到电脑上或在打印机上打印出来。 

应用 

动物细胞融合

核转移

胚胎操作

杂交瘤生成

植物原生质体融合

活体/离体/卵内基因或drug导入

动物细胞或组织的转染

干细胞生成

部分原核生物（bacteria）、酵母的转化              

已发表文献：

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Wang K, et al. Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-Directed Repair. Mol TherNucleic Acids, 2016 Nov;5(11): e396.

Mangan, PR, et al. Dual inhibition of interleukin-23 and interleukin-17 offers superior efficacy in mouse models of autoimmunity. J Pharmacol Exp Ther. 2015 Aug;354(2), 152-165.

Ruiz N, et al. Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation. PLoS One. 2015 Jun; 10(6): e0130008.

Wang, K, et al. Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system. Sci Rep. 2015 Nov;13(5): 16623.

Wang Y, et al. MiR-499 Enhances the Cisplatin Sensitivity of Esophageal Carcinoma Cell Lines by Targeting DNA Polymerase β. Cell Physiol Biochem. 2015;36: 1587-1596.

Wu H, et al. TALE Nickase-Mediated SP110 Knockin Endows Cattle with Increased Resistance to Tuberculosis. Proc Natl Acad Sci USA. 2015 Mar;112(13): E1530-1539.

Zhang XH, Lian XD, Dai ZX, Zheng HY, Chen X, Zheng YT. α3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2015 Sep;199(6) :2030-2042.

Lai CW, et al. FTSJ2, a Heat Shock-Inducible Mitochondrial Protein, Suppresses Cell Invasion and Migration. PLoS One. 2014 Mar; 9(3): e90818.

Zhang Y, Luo W, Wang Y, Liu Y, and Zheng, L. Purified dendritic cell-tumor fusion hybrids supplemented with non-adherent dendritic cells fraction are superior activators of antitumor immunity. PLoS One, 2014;9(1), e86772.

Liu X, et al. Zinc-Finger Nickase-Mediated Insertion of the Lysostaphin Gene into the Beta-Casein Locus in Cloned Cows. Nat Comm. 2013;4: 2565.

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