产品详情
简单介绍：
dCas9 C-terminal Cloning Vector
详情介绍：
| Print Version | |
| Description | This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal. |
| Product Application | |
| Species | N/A |
| Vector | pLenti-EF1a-dCas9-Cloning |
| Bacterial Selection | Kanamycin |
| Format | Plasmid |
| Appearance | Liquid |
| Storage | -20°C or below |
| Shelf Life | 1 year (when at -20°C or below in a non-frost free freezer) |
| Shipping | Shipped at ambient temperature |
| Additional Materials | - CRISPR Genomic Cleavage Detection Kit (G932) - sgRNA CRISPR Lentivector Control (K018) |
| Quality Control | Restriction Enzyme Digest and Sequencing |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
| Note | abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant). |
| Disclaimer | 1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials. |
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