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  • 入驻时间: 2008-08-20
  • 联系人:客户经理
  • 电话:400-968-7988
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  • Email:fuwu@bioleaf.com
产品详情
  • 产品名称:T4 DNA Polymerase

  • 产品型号:E005
  • 产品厂商:ABM
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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简单介绍:
T4 DNA Polymerase
详情介绍:
Print Version
Description T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
Application
  • Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs
  • High fidelity due to strong 3’→5’ exonuclease activity
  • Synthesis of labeled DNA probes by the replacement reaction
  • Site-specific mutagenesis via primer extension from oligonucleotides
  • Form Enzyme supplied with 10X Reaction Buffer
    Image
    Concentration 5 U/μl
    Storage Store all components at -20°C.
    Storage Buffer 100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
    Notes One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.
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