产品详情
简单介绍：
Phi29 DNA Polymerase
详情介绍：
| Print Version | |
| Description | Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates. |
| Application | • Rolling circle amplification (RCA) • Multiple displacement amplification (MDA) • Whole genome amplification (WGA) • DNA template preparation for sequencing • Protein-primed DNA amplification |
| Form | Enzyme supplied with 5X Reaction Buffer |
| Image |  |
| Concentration | 10 U/μl |
| Storage | Store all components at -20°C. |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol. |
| Notes | One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use . |
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