产品详情
简单介绍：
Pat5 Embryonic Stem Cells
详情介绍：
| Print Version | |
| Safety | BioSafety Level II |
| Organism | 129X1/SvJ mouse |
| Source Organ | Embryo |
| Description | Pat5 Embryonic Stem Cells were derived from 129X1/SvJ mouse embryos and can be utilized to introduce genetic mutations with gene targeting vectors through homologous recombination. Growth Properties: Adherent Morphology: Polygonal |
| Concentration | 106cells/vial |
| Propagation | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. Cell line must be maintained on γ-irradiated mouse embryonic fibroblasts (T2003). The base medium for this cell line is Prigrow III medium available in abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. |
| Storage | -180°C |
| Shipping | On Dry Ice |
| Notes | For Research Use Only, not for therapeutic or diagnostic purposes. |
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