产品详情
简单介绍：
EF1α Promoter mYamanaka Factors Lentivirus Set
详情介绍：
| Print Version | |
| Vector | Refer to Individual Virus Pages |
| Plasmid Size | Refer to individual viruses. |
| Promoter | EF1α |
| Gene | mOct4, mSox2, mKLF4, mMyc and GFP control virus. |
| Organism | Mouse |
| Accession # | P20263, P48432, Q60793 and P01108 |
| Description | Five Lenti-III-EF1α viruses, expressing the Yamanaka iPSC factor set (mOct4, mSox2, mKLF4 and mMyc) and a GFP control. Purified ready-to-use high titer lentivirus at 1x107 cfu/mL |
| Functions | Each individual recombinant lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors provided in this set in combinantion with each other has been shown to reprogram ***** mouse fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs). |
| Quality Control | Viral titer (IU/ml) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABM's viral preparations are tested to be free of bacteria and other microbials. |
| Titer | 1x107 IU/ml |
| Storage | -70°C |
| Shipping | On Dry Ice |
| Notes | For Research Use Only, not for therapeutic or diagnostic purposes. |
| References | Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar Induction of pluripotent stem cells from ***** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and ***** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. |
相关文章
