产品详情
简单介绍：
EF1α Polycistronic mYamanaka Factors Lentivirus
详情介绍：
| Print Version | |
| Vector | pLenti-III-EF1α-Polytronic-mOSKMG |
| Promoter | EF1α |
| Gene | mOct4, mSox2, mKLF4, mMyc and GFP |
| Organism | Mouse |
| Accession # | P20263, P48432, Q60793 and P01108 |
| Description | The polycistronic lentivirus expresses all of the four mouse transcription factors (mOSKM of Yamanaka set: mOct4, mSox2, mKLF4 or mMyc) within a single expression cassette under the control of EF1α promoter. Each of the factor's ORF is separated by a unique 2A peptide (P2A, T2A, and E2A) sequence, which allows for the equivalent expression of all four transcription factors from a single vector. The expression of all factors from a single virus minimizes the number of proviral integrations required for successful reprogramming. This also reduces the risk of insertional mutagenesis effects in the resultant induced pluripotent stem (iPS) cells when compared to those generated using four independent viruses. Also, the virus is provided as concentrated and VSV-G pseudotyped stock capable of transducing both dividing and non-dividing cells. A control GFP lentivirus is included for the easy tracking of gene transduction efficiency in your target cells. |
| Functions | The recombinant polycistronic lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of these transcription factors has been shown to reprogram ***** mouse fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs). |
| Quality Control | Viral titer (IU/ml) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABM's viral preparations are tested to be free of bacteria and other microbials. |
| Titer | 1x107 IU/ml |
| Storage | -70°C |
| Shipping | On Dry Ice |
| Notes | For Research Use Only, not for therapeutic or diagnostic purposes. |
| References | Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar Induction of pluripotent stem cells from ***** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and ***** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. |
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