产品详情
简单介绍：
Yamanaka Factor Polycistronic Adenovirus (Human)
详情介绍：
| Print Version | |
| Vector | pAdeno |
| Promoter | CMV |
| Insert | Yamanaka Factor Polycistronic |
| Picture |  |
| Organism | Human |
| Accession # | Refer to individual adenovirus. |
| Alias | Oct4, Sox2, KLF4 and Myc |
| Functions | Yamanaka iPSC factors (Oct4, Sox2, KLF4 and Myc) expressed from a single CMV promoter in a single adenoviral vector. All four factors are linked by protein 2A sequence which allows similar levels of transgene expression of four factors in target cells, thus streamlining iPSC reprogramming. A GFP control adenovirus is included for easy tracking of gene transduction efficiency. |
| Gene Type | cDNA |
| Purification | By Ion-Exchange column. |
| Quality Control | By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming. |
| Titer | >1x106pfu/mL |
| Storage | 10mM Tris-HCl, pH 8.0. 2.5% glycerol |
| Shipping | High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Notes | For Research Use Only, not for therapeutic or diagnostic purposes. |
| References | Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1. |
相关文章
