产品详情
简单介绍：
mYamanaka Factor Set Adenovirus (Mouse)
详情介绍：
| Print Version | |
| Vector | pAdeno |
| Promoter | CMV |
| Insert | mYamanaka Factor Set |
| Picture |  |
| Organism | Mouse |
| Accession # | Refer to individual adenovirus. |
| Alias | mOct4, mSox2, mKLF4, mMyc and GFP control virus. |
| Protein Type | Wild Type |
| Functions | Co-expression of mOct4, mSox2, mKLF4 and mMyc (Yamanaka set) in any somatic cell will lead to iPSC reprogramming. The Yamanaka set of transcription factors provides purified, high titer adenoviruses that offer the highest gene transduction efficiency for iPSC induction. The adenoviral vector will not integrated into host cell genome, avoiding possible mutations associated with vector insertion. |
| Gene Type | cDNA |
| Purification | By Ion-Exchange column. |
| Quality Control | By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming. |
| Titer | >1x106pfu/mL |
| Storage | 10 mM Tris-HCl, pH 8.0 |
| Shipping | High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Notes | For Research Use Only, not for therapeutic or diagnostic purposes. |
| References | Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1. |
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