产品详情
简单介绍：
EACH-1 Cells
详情介绍：
| Print Version | |
| BioSafety Level | II |
| Organism | Human |
| Source Organ | Posterior scapular spine |
| Disease | Parachordoma |
| Donor Age | 28 |
| Donor Gender | Male |
| Growth Properties | Adherent |
| Morphology | Polygonal |
| Population Doubling | Doubling time of 37 hours |
| Recommended Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Markers | Vimentin, S-100, AE1/AE3, p63 (negative) |
| Applications | For Research Use Only |
| Description | EACH-1 Cells are novel extra-axial chordoma tumor cells which exhibit chromosomal aberrations, and are tumorigenic and can form colonies on soft agar. It may be used to facilitate studies on the development and treatment of the rare low grade malignant tumor. |
| Procedure Overview |  |
| Propagation | The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. |
| Quality Control | Flow cytometry was used to identify cell surface markers |
| Reference | Decomas, A. M., Penfornis, P., Harris, M. R., Meyer, M. S., & Pochampally, R. R. (2010). Derivation and Characterization of an Extra-Axial Chordoma Cell Line (EACH-1) from a Scapular Tumor. The Journal of Bone and Joint Surgery-American Volume, 92(5), 1231-1240. doi:10.2106/jbjs.i.00594 |
| Disclaimer | 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
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