产品详情
简单介绍：
IU-TAB-1 Cells
详情介绍：
| Print Version | |
| BioSafety Level | II |
| Organism | Human |
| Source Organ | Thymic |
| Disease | Thymoma (AB type) |
| Donor Age | 53 |
| Donor Gender | Male |
| Growth Properties | Adherent |
| Morphology | Polygonal |
| Population Doubling | 48 hours |
| Recommended Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Markers | Epithelial markers (pan-cytokeratin and EpCAM/CD326), thymic epithelial surface markers (CD29, CD9, CD54/ICAM-1, CD58 and CD24), and p63 |
| Applications | For Research Use Only |
| Description | IU-TAB-1 Cells are established in vitro through the isolation from tissue derived from patient with stage II thymoma, classified as type AB. These cells are able to proliferate in culture and are valuable for research in thymic malignancies. This is particularly useful in studies relating to the cellular and molecular mechanisms of cancer biology in thymomas. |
| Procedure Overview |  |
| Propagation | Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow II medium available from abm (TM002). To make the completed growth medium, add the following components to the base medium with the final concentration: 10% fetal bovine serum (TM999) and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. |
| Quality Control | Immunophenotype and FCS analysis for cell markers |
| Disclaimer | 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
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