LOCUS Exported 7507 bp ds-DNA circular SYN 19-MAY-2017
DEFINITION Single-copy E. coli plasmid vector designed for constructing
Bacterial Artificial Chromosomes (BACs).
ACCESSION U51113
VERSION .
KEYWORDS pBeloBAC11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7507)
AUTHORS New England Biolabs
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7507
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
CDS complement(56..436)
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLL
THLCGISHRIWCTLSTICSDAA"
primer_bind 289..305
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 312..330
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 333..389
/note="MCS"
/note="pUC18/19 multiple cloning site"
promoter complement(396..414)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(432..448)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 456..472
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(480..510)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
CDS complement(766..1425)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(1426..1528)
/note="cat promoter"
/note="promoter of the E. coli cat gene"
rep_origin 2455..2674
/note="ori2"
/note="secondary origin of replication for the bacterial F
plasmid; also known as oriS"
CDS 2765..3520
/codon_start=1
/gene="repE"
/product="replication initiation protein for the bacterial
F plasmid"
/note="repE"
/translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
misc_feature 3523..3773
/gene="incC"
/note="incC"
/note="incompatibility region of the bacterial F plasmid"
CDS 4099..5274
/codon_start=1
/gene="sopA"
/product="partitioning protein for the bacterial F plasmid"
/note="sopA"
/translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
CDS 5274..6245
/codon_start=1
/gene="sopB"
/product="partitioning protein for the bacterial F plasmid"
/note="sopB"
/translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
misc_feature 6318..6791
/gene="sopC"
/note="sopC"
/note="centromere-like partitioning region of the bacterial
F plasmid"
misc_feature 7051..7449
/note="cos"
/note="lambda cos site; allows packaging into phage lambda
particles"
protein_bind 7467..7500
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
ORIGIN
1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
181 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
241 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
301 ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cggggatcct
361 ctagagtcga cctgcaggca tgcaagcttg agtattctat agtgtcacct aaatagcttg
421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
601 cattaatgaa tcggccaacg cgaacccctt gcggccgccc gggccgtcga ccaattctca
661 tgtttgacag cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc
721 gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg
781 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
841 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
901 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
961 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
1021 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
1081 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
1141 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
1201 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
1261 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
1321 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
1381 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
1441 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
1501 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
1561 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
1621 aggtatttat tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga
1681 aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg
1741 ccgccgctgc tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc
1801 attcctatgc gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga
1861 tgggacataa gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg
1921 ctgcccggca ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac
1981 aattatcctg agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg
2041 ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag
2101 tcgggaaaat ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc
2161 gtttatagga agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat
2221 atgccttcag gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg
2281 tcagcaatgg acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca
2341 gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc ctcgagtgag cgaggaagca
2401 ccagggaaca gcacttatat attctgctta cacacgatgc ctgaaaaaac ttcccttggg
2461 gttatccact tatccacggg gatattttta taattatttt ttttatagtt tttagatctt
2521 cttttttaga gcgccttgta ggcctttatc catgctggtt ctagagaagg tgttgtgaca
2581 aattgccctt tcagtgtgac aaatcaccct caaatgacag tcctgtctgt gacaaattgc
2641 ccttaaccct gtgacaaatt gccctcagaa gaagctgttt tttcacaaag ttatccctgc
2701 ttattgactc ttttttattt agtgtgacaa tctaaaaact tgtcacactt cacatggatc
2761 tgtcatggcg gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt
2821 ccagtcaaac gacctcactg aggcggcata tagtctctcc cgggatcaaa aacgtatgct
2881 gtatctgttc gttgaccaga tcagaaaatc tgatggcacc ctacaggaac atgacggtat
2941 ctgcgagatc catgttgcta aatatgctga aatattcgga ttgacctctg cggaagccag
3001 taaggatata cggcaggcat tgaagagttt cgcggggaag gaagtggttt tttatcgccc
3061 tgaagaggat gccggcgatg aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc
3121 gcacagtcca tccagagggc tttacagtgt acatatcaac ccatatctca ttcccttctt
3181 tatcgggtta cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa
3241 tccgtatgcc atgcgtttat acgaatccct gtgtcagtat cgtaagccgg atggctcagg
3301 catcgtctct ctgaaaatcg actggatcat agagcgttac cagctgcctc aaagttacca
3361 gcgtatgcct gacttccgcc gccgcttcct gcaggtctgt gttaatgaga tcaacagcag
3421 aactccaatg cgcctctcat acattgagaa aaagaaaggc cgccagacga ctcatatcgt
3481 attttccttc cgcgatatca cttccatgac gacaggatag tctgagggtt atctgtcaca
3541 gatttgaggg tggttcgtca catttgttct gacctactga gggtaatttg tcacagtttt
3601 gctgtttcct tcagcctgca tggattttct catacttttt gaactgtaat ttttaaggaa
3661 gccaaatttg agggcagttt gtcacagttg atttccttct ctttcccttc gtcatgtgac
3721 ctgatatcgg gggttagttc gtcatcattg atgagggttg attatcacag tttattactc
3781 tgaattggct atccgcgtgt gtacctctac ctggagtttt tcccacggtg gatatttctt
3841 cttgcgctga gcgtaagagc tatctgacag aacagttctt ctttgcttcc tcgccagttc
3901 gctcgctatg ctcggttaca cggctgcggc gagcgctagt gataataagt gactgaggta
3961 tgtgctcttc ttatctcctt ttgtagtgtt gctcttattt taaacaactt tgcggttttt
4021 tgatgacttt gcgattttgt tgttgctttg cagtaaattg caagatttaa taaaaaaacg
4081 caaagcaatg attaaaggat gttcagaatg aaactcatgg aaacacttaa ccagtgcata
4141 aacgctggtc atgaaatgac gaaggctatc gccattgcac agtttaatga tgacagcccg
4201 gaagcgagga aaataacccg gcgctggaga ataggtgaag cagcggattt agttggggtt
4261 tcttctcagg ctatcagaga tgccgagaaa gcagggcgac taccgcaccc ggatatggaa
4321 attcgaggac gggttgagca acgtgttggt tatacaattg aacaaattaa tcatatgcgt
4381 gatgtgtttg gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt gatcggggtt
4441 gctgcccata aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg
4501 gctctgaagg ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg aacagcctca
4561 atgtatcacg gatgggtacc agatcttcat attcatgcag aagacactct cctgcctttc
4621 tatcttgggg aaaaggacga tgtcacttat gcaataaagc ccacttgctg gccggggctt
4681 gacattattc cttcctgtct ggctctgcac cgtattgaaa ctgagttaat gggcaaattt
4741 gatgaaggta aactgcccac cgatccacac ctgatgctcc gactggccat tgaaactgtt
4801 gctcatgact atgatgtcat agttattgac agcgcgccta acctgggtat cggcacgatt
4861 aatgtcgtat gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt gtttgactac
4921 acctccgcac tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa
4981 gggttcgagc ctgatgtacg tattttgctt accaaataca gcaatagtaa tggctctcag
5041 tccccgtgga tggaggagca aattcgggat gcctggggaa gcatggttct aaaaaatgtt
5101 gtacgtgaaa cggatgaagt tggtaaaggt cagatccgga tgagaactgt ttttgaacag
5161 gccattgatc aacgctcttc aactggtgcc tggagaaatg ctctttctat ttgggaacct
5221 gtctgcaatg aaattttcga tcgtctgatt aaaccacgct gggagattag ataatgaagc
5281 gtgcgcctgt tattccaaaa catacgctca atactcaacc ggttgaagat acttcgttat
5341 cgacaccagc tgccccgatg gtggattcgt taattgcgcg cgtaggagta atggctcgcg
5401 gtaatgccat tactttgcct gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc
5461 ggggtgatag tgttgagaag acctctcggg tatggtcagg taatgaacgt gaccaggagc
5521 tgcttactga ggacgcactg gatgatctca tcccttcttt tctactgact ggtcaacaga
5581 caccggcgtt cggtcgaaga gtatctggtg tcatagaaat tgccgatggg agtcgccgtc
5641 gtaaagctgc tgcacttacc gaaagtgatt atcgtgttct ggttggcgag ctggatgatg
5701 agcagatggc tgcattatcc agattgggta acgattatcg cccaacaagt gcttatgaac
5761 gtggtcagcg ttatgcaagc cgattgcaga atgaatttgc tggaaatatt tctgcgctgg
5821 ctgatgcgga aaatatttca cgtaagatta ttacccgctg tatcaacacc gccaaattgc
5881 ctaaatcagt tgttgctctt ttttctcacc ccggtgaact atctgcccgg tcaggtgatg
5941 cacttcaaaa agcctttaca gataaagagg aattacttaa gcagcaggca tctaaccttc
6001 atgagcagaa aaaagctggg gtgatatttg aagctgaaga agttatcact cttttaactt
6061 ctgtgcttaa aacgtcatct gcatcaagaa ctagtttaag ctcacgacat cagtttgctc
6121 ctggagcgac agtattgtat aagggcgata aaatggtgct taacctggac aggtctcgtg
6181 ttccaactga gtgtatagag aaaattgagg ccattcttaa ggaacttgaa aagccagcac
6241 cctgatgcga ccacgtttta gtctacgttt atctgtcttt acttaatgtc ctttgttaca
6301 ggccagaaag cataactggc ctgaatattc tctctgggcc cactgttcca cttgtatcgt
6361 cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
6421 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac
6481 tcgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga
6541 ttattagtct gggaccatgg tcccactcgt atcgtcggtc tgattattag tctgggacca
6601 cggtcccact cgtatcgtcg gtctgattat tagtctggaa ccacggtccc actcgtatcg
6661 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
6721 ctgggaccac gatcccactc gtgttgtcgg tctgattatc ggtctgggac cacggtccca
6781 cttgtattgt cgatcagact atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg
6841 caagtattga catgtcgtcg taacctgtag aacggagtaa cctcggtgtg cggttgtatg
6901 cctgctgtgg attgctgctg tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg
6961 acaaaatacc tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag
7021 tgtgtcgctg tcgacgagct cgcgagctcg gacatgaggt tgccccgtat tcagtgtcgc
7081 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata
7141 cctgcgtcat aattgattat ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt
7201 agatgataat cattatcact ttacgggtcc tttccggtga tccgacaggt tacggggcgg
7261 cgacctcgcg ggttttcgct atttatgaaa attttccggt ttaaggcgtt tccgttcttc
7321 ttcgtcataa cttaatgttt ttatttaaaa taccctctga aaagaaagga aacgacaggt
7381 gctgaaagcg agctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga
7441 acaatggaag tccgagctca tcgctaataa cttcgtatag catacattat acgaagttat
7501 attcgat
//