LOCUS Exported 2831 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..2831
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 70..92
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 100..132
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
misc_feature 141..187
/label=MCS
terminator 246..293
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 390..845
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 871..975
/gene="bla"
/label=AmpR promoter
CDS 976..1836
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2007..2595
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tggctagcat gactggtgga
121 cagcaaatgg gtcgcggatc aagcttcgaa ttccatggta ccagctgcag atctcgagct
181 cggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
241 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
301 gaggaactat atccggatct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
361 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
421 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
481 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
541 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
601 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
661 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
721 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
781 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
841 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
1021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
1081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
1141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
1201 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
1261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
1321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
1381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
1441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
1501 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
1561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
1621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
1681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
1741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
1801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
1861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
1921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
1981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
2041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
2101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
2161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
2221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
2341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
2401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
2461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
2521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
2581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
2641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
2701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
2761 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
2821 cattaatgca g
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