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产品名称：pQE-30 UA (linearized)
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简单介绍

pQE-30 UA (linearized)菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pQE-30 UA (linearized)收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

产品描述

pQE-30 UA (linearized)

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0621	pQE-30 UA (linearized)	20μl	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T5
复制子:	ColE1 ori
终止子:	Lambda t0 terminator；rrnB T1
质粒分类:	大肠杆菌载体；pQE系列表达质粒
质粒大小:	3503bp
原核抗性:	氨苄青霉素Amp
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	M15
培养条件:	37℃，有氧，LB
诱导方式:	IPTG或乳糖及其类似物
5'测序引物:	pQE30-F: AGCGGATAACAATTTCACACAG
3'测序引物:	pQE30-R: TTCTGAGGTCATTACTGGATC

质粒简介

质粒图谱

质粒序列

LOCUS       Exported                3504 bp ds-DNA     linear   SYN 03-JAN-2014
DEFINITION Linearized bacterial expression and 6xHis-tagging vector with 3'-U
            overhangs for UA cloning of a PCR product.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-30 UA (linearized)
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 3504)
AUTHORS   Qiagen
TITLE     Direct Submission
JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     Linearized for UA cloning.
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    2..61
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    61..71
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      77..171
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             215..874
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      939..1025
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1506..2094)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2265..3125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3126..3230)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3340..3384
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    3360..3376
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3392..3408
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3431..3436
                     /note="ribosome binding site"
     CDS             3445..3447
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             3457..3474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     misc_feature    3475..3503
                     /note="MCS"
                     /note="multiple cloning site"
ORIGIN
        1 agaagcgatt gaggagatct gagctcggta cccgggtcga caggcctctg cagccaagct
       61 taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      121 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag
      181 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
      241 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      301 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
      361 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
      421 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
      481 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
      541 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
      601 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
      661 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
      721 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
      781 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
      841 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
      901 ttaaacgcct ggggtaatga ctctctagct tgaggcatca aataaaacga aaggctcagt
      961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1021 caaatccgcc ctctagagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     1081 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     1141 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     1201 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1261 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     1321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1921 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     1981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3241 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3301 aggcgtatca cgaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt
     3361 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa
     3421 ttcattaaag aggagaaatt aactatgaga ggatcgcatc accatcacca tcacggatcc
     3481 cacgtgatat cctcaatcgc ttct
//

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产品资料

pQE-30 UA (linearized)

pQE-30 UA (linearized)

产品信息

使用说明

基本信息

质粒简介

质粒图谱

质粒序列