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pQE-51

pQE-51
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  • 产品名称：pQE-51
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简单介绍
pQE-51菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pQE-51收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
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pQE-51

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0629 pQE-51

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: T5
复制子: ColE1 ori
终止子: Lambda t0 terminator；rrnB T1
质粒分类: 大肠杆菌载体；pQE系列表达质粒
质粒大小: 3437bp
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: M15
培养条件: 37℃，有氧，LB
诱导方式: IPTG或乳糖及其类似物
5'测序引物: pQE30-F: AGCGGATAACAATTTCACACAG
3'测序引物: pQE30-R: TTCTGAGGTCATTACTGGATC

质粒简介

质粒图谱

质粒序列

LOCUS       Exported                3437 bp ds-DNA     circular SYN 05-DEC-2013
DEFINITION  Bacterial vector for expressing untagged proteins. For other reading
            frames, use pQE-50 or pQE-52.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-51
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Qiagen
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     misc_feature    120..167
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    167..177
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      183..277
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             321..980
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1045..1131
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1613..2201)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2372..3232)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3233..3337)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgagg
      121 gatccgcatg cgagctcggt accccgggtc gacctgcagc caagcttaat tagctgagct
      181 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
      241 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt
      301 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      361 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      421 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      481 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      541 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      601 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      661 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      721 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      781 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      841 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      901 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
      961 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg
     1021 taatgactct ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1081 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgctc
     1141 tagagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     1201 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     1261 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     1321 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     1381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     1441 cctcgctcac tgactcgctg cgctcggtct gtcggctgcg gcgagcggta tcagctcact
     1501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1801 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2041 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2401 tctcagcgat ctgtctattt cgttcatcca tagctgcctg actccccgtc gtgtagataa
     2461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3421 ggccctttcg tcttcac
//
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