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pQE-TriSystem Strep

pQE-TriSystem Strep
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  • 产品名称：pQE-TriSystem Strep
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简单介绍
pQE-TriSystem Strep菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pQE-TriSystem Strep收到质粒后请短暂��心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
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pQE-TriSystem Strep

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0650 pQE-TriSystem Strep

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: p10
复制子: ColE1 ori
终止子: T7 terminator
质粒分类: 大肠杆菌载体；pQE系列表达质粒
质粒大小: 5736bp
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: M15
培养条件: 37℃，有氧，LB
诱导方式: IPTG或乳糖及其类似物

质粒简介

质粒图谱

质粒序列

LOCUS       Exported                5736 bp ds-DNA     circular SYN 05-MAY-2014
DEFINITION  Vector for expression in bacterial, insect, and vertebrate cells of
            proteins with a C-terminal Strep-Tag II tag.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem Strep
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Qiagen
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        62..367
                     /note="CMV enhancer"
                     /note="human cytomegalovirus (CMV) immediate early
                     enhancer"
     promoter        373..650
                     /note="chicken beta-actin promoter"
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     exon            1005..1589
                     /note="Exon 2"
     promoter        1073..1116
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    1092..1108
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1143..1252
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     misc_feature    1265..1372
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1267..1269
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             1381..1404
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin, an engineered
                     form of streptavidin"
                     /note="Strep-Tag II"
                     /translation="WSHPQFEK"
     misc_feature    1411..1421
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1565..1570
     terminator      1587..1698
                     /note="rabbit globin terminator"
     terminator      1708..1755
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     misc_recomb     1768..3226
                     /note="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     CDS             complement(1885..3226)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /note="ORF1629"
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      complement(3385..3972)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3985..4845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_recomb     4911..2
                     /note="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     CDS             4911..5035
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /note="lef2"
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5073..5678)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /note="ORF603"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
      661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
      781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
     1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
     1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
     1261 tataccatgg cgatatcccg ggagctcgtg gatccgaatt ctcagatctc ggcgcgcctg
     1321 caggtcgacg gtaccggttc gaagcttgcg gccgcacagc tgtatacacg tgcaagcgcg
     1381 tggagccacc cgcagttcga aaaataacac taagtgatta acctcaggtg caggctgcct
     1441 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
     1501 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1561 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1621 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagtt tttggtttag
     1681 agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc taaacgggtc
     1741 ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt ccctggtgtt
     1801 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     1861 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     1921 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     1981 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     2041 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     2101 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     2161 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     2221 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     2281 aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     2341 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     2401 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     2461 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
     2521 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
     2581 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
     2641 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
     2701 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
     2761 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
     2821 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
     2881 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
     2941 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
     3001 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
     3061 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
     3121 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
     3181 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcggaacgc tgcgctcggt
     3241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3301 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
     3421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3961 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
     4021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4861 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtttcc tgcatctttt
     4921 aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa
     4981 gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt attgaataat
     5041 aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa cgcaacaaga
     5101 acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag gtaatattta
     5161 aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt ttcacataaa
     5221 ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt ctcttcataa
     5281 aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt aaattttttg
     5341 ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg catagttttt
     5401 ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc tttaattatt
     5461 aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt gccggcatag
     5521 tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt aacataactt
     5581 tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc tatactattg
     5641 tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg cacaaactaa
     5701 tatcacaaac tggaaatgtc tatcaatata tagttg
//
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