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产品名称：pQE-7
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简单介绍

pQE-7菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pQE-7收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

产品描述

pQE-7

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0605	pQE-7	20μl	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T5
复制子:	ColE1 ori
终止子:	Lambda t0 terminator；rrnB T1
质粒分类:	大肠杆菌载体；pQE系列表达质粒
质粒大小:	3416bp
原核抗性:	氨苄青霉素Amp
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	M15
培养条件:	37℃，有氧，LB
诱导方式:	IPTG或乳糖及其类似物
5'测序引物:	pQE30-F: AGCGGATAACAATTTCACACAG
3'测序引物:	pQE30-R: TTCTGAGGTCATTACTGGATC

质粒简介

质粒图谱

质粒序列

LOCUS       Exported                3416 bp ds-DNA     circular SYN 03-DEC-2013
DEFINITION Bacterial expression vector with a T5 promoter. For alternative
            bases flanking the start codon, use pQE-6.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-7
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 3416)
AUTHORS   Qiagen
TITLE     Direct Submission
JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     misc_feature    121..146
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    146..156
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      162..256
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             300..959
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1024..1110
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1592..2180)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2351..3211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3212..3316)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aagcatgcga
      121 ggatccgtcg acctgcagcc aagcttaatt agctgagctt ggactcctgt tgatagatcc
      181 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      241 ttttattggt gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa
      301 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      361 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      421 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      481 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
      541 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      601 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      661 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      721 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      781 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      841 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
      901 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
      961 ttttttaagg cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg
     1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1081 tcggtgaacg ctctcctgag taggacaaat ccgccgctct agagctgcct cgcgcgtttc
     1141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1261 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     1321 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1381 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1441 gctcggtctg tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     1801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2401 gttcatccat agctgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3361 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac
//

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产品目录

产品资料

pQE-7

pQE-7

产品信息

使用说明

基本信息

质粒简介

质粒图谱

质粒序列