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pQE-5

pQE-5
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  • 产品名称：pQE-5
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简单介绍
pQE-5菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pQE-5收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
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pQE-5

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0603 pQE-5

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: T5
终止子: Lambda t0 terminator；rrnB T1
质粒分类: 大肠杆菌载体；pQE系列表达质粒
质粒大小: 3414bp
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: M15
培养条件: 37℃，有氧，LB
诱导方式: IPTG或乳糖及其类似物
5'测序引物: pQE30-F: AGCGGATAACAATTTCACACAG
3'测序引物: pQE30-R: TTCTGAGGTCATTACTGGATC

质粒简介

质粒图谱

质粒序列

LOCUS       Exported                3414 bp ds-DNA     circular SYN 03-DEC-2013
DEFINITION  Bacterial expression vector with a T5 promoter. For other reading
            frames, use pQE-3 or pQE-4.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Qiagen
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     misc_feature    119..144
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    144..154
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      160..254
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             298..957
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1022..1108
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1590..2178)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2349..3209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3210..3314)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgagg
      121 atccgtcgac ctgcagccaa gcttaattag ctgagcttgg actcctgttg atagatccag
      181 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
      241 ttattggtga gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg
      301 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      361 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      421 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      481 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
      541 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      601 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      661 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      721 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      781 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
      841 aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc
      901 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
      961 ttttaaggca gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca
     1021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1081 ggtgaacgct ctcctgagta ggacaaatcc gccgctctag agctgcctcg cgcgtttcgg
     1141 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     1201 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     1261 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     1321 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     1381 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1441 tcggtctgtc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2221 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2281 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2341 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2401 tcatccatag ctgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2461 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2521 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2581 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2641 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2701 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2761 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2821 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2881 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2941 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3001 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3061 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3121 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3181 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3361 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//
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