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pQE-4

pQE-4
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  • 产品名称：pQE-4
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简单介绍
pQE-4菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pQE-4收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
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pQE-4

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0602 pQE-4

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: T5
终止子: Lambda t0 terminator；rrnB T1
质粒分类: 大肠杆菌载体；pQE系列表达质粒
质粒大小: 3415bp
原核抗性: 氨苄青霉素Amp
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: M15
培养条件: 37℃，有氧，LB
诱导方式: IPTG或乳糖及其类似物
5'测序引物: pQE30-F: AGCGGATAACAATTTCACACAG
3'测序引物: pQE30-R: TTCTGAGGTCATTACTGGATC

质粒简介

质粒图谱

质粒序列

LOCUS       Exported                3415 bp ds-DNA     circular SYN 03-DEC-2013
DEFINITION  Bacterial expression vector with a T5 promoter. For other reading
            frames, use pQE-3 or pQE-5.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Qiagen
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     misc_feature    120..145
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    145..155
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      161..255
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             299..958
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1023..1109
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1591..2179)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2350..3210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3211..3315)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgagg
      121 gatccgtcga cctgcagcca agcttaatta gctgagcttg gactcctgtt gatagatcca
      181 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
      241 tttattggtg agaatccaag ctagcttggc gagattttca ggagctaagg aagctaaaat
      301 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      361 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      421 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
      481 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
      541 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
      601 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
      661 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
      721 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
      781 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
      841 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt
      901 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
      961 tttttaaggc agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc
     1021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1081 cggtgaacgc tctcctgagt aggacaaatc cgccgctcta gagctgcctc gcgcgtttcg
     1141 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     1201 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     1261 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     1321 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     1381 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1441 ctcggtctgt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     1801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2401 ttcatccata gctgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3361 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcac
//
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