LOCUS Exported 11160 bp ds-DNA circular SYN 09-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11160
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..861
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 868..1494
/codon_start=1
/gene="tetR"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
CDS complement(1536..2486)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 2534..2756
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
promoter 3300..3355
/gene="tetR"
/label=tetR/tetA promoters
/note="overlapping promoters for bacterial tetR and tetA"
protein_bind 3306..3324
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
protein_bind 3336..3354
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 3403..4674
/codon_start=1
/product="site-specific recombinase"
/label=FLP
/note="FLP is a site-specific recombinase from
Saccharomyces cerevisiae. Recombination occurs at FRT
sequences."
/translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
THNGTAIKRATFMSYNTIISNSLSLDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
YPAWNGIISQEVLDYLSSYINRRI"
CDS complement(4836..5072)
/codon_start=1
/gene="dhfrIIb from plasmid R388"
/product="E. coli plasmid-associated dihydrofolate
reductase"
/label=TpR
/note="confers resistance to trimethoprim"
/translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
promoter complement(5399..5427)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
CDS complement(5458..6306)
/codon_start=1
/gene="E. coli rhaR"
/product="transcriptional activator of rhaR and rhaS"
/label=rhaR
/note="needed for rhamnose-dependent activation of the rhaB
promoter"
/translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
D"
CDS complement(6380..7216)
/codon_start=1
/gene="E. coli rhaS"
/product="positive regulator of the rhaB promoter"
/label=rhaS
/note="activated by L-rhamnose"
/translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
promoter 7362..7480
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
CDS 7504..7920
/codon_start=1
/gene="gam"
/product="inhibitor of the host RecBCD nuclease in the
lambda Red system"
/label=Gam
/note="protects the double-stranded DNA substrate for
recombination"
/translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
CDS 7926..8711
/codon_start=1
/gene="bet"
/product="single-stranded DNA binding recombinase in the
lambda Red system"
/label=Beta
/note="mediates pairing of a single-stranded DNA with a
complementary target"
/translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
CDS 8708..9388
/codon_start=1
/gene="exo"
/product="5' to 3' double-stranded DNA exonuclease in the
lambda Red system"
/label=Exo
/note="creates 3' single-stranded DNA tails"
/translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
EQWR"
CDS 9412..10473
/codon_start=1
/gene="recA"
/product="DNA repair protein from E. coli"
/label=RecA
/translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGS
LSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIY
ARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDS
HMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASV
RLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVK
EKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSV
DDSEGVAETNEDF"
rep_origin 10574..11029
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 11056..11160
/gene="bla"
/label=AmpR promoter
ORIGIN
1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
601 tcccggcaac aattgataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct
721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
841 tcactgatta agcattggta ggaattaatg atgtctcgtt tagataaaag taaagtgatt
901 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
961 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
1021 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
1081 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
1141 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
1201 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
1261 ttatatgcac tcagcgcagt ggggcatttt actttaggtt gcgtattgga agatcaagag
1321 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
1381 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
1441 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc ttaaaagcag
1501 cataaccttt ttccgtgatg gtaacttcag ctagctcaga tccttccgta tttagccagt
1561 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
1621 tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
1681 gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg
1741 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
1801 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
1861 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
1921 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
1981 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
2041 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
2101 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
2161 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
2221 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
2281 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
2341 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
2401 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
2461 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
2521 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
2581 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
2641 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
2701 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
2761 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
2821 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
2881 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
2941 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
3001 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
3061 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
3121 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
3181 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtctagac
3241 tcgaggaatt gatatcaccc gacaccatcg aatggccaga tgattaattc ctaatttttg
3301 ttgacactct atcattgata gagttatttt accactccct atcagtgata gagaaaagtg
3361 aaatgaatag ttcgacaaaa atctagataa cgagggcaaa aaatgccaca atttgatata
3421 ttatgtaaaa caccacctaa ggtgcttgtt cgtcagtttg tggaaaggtt tgaaagacct
3481 tcaggtgaga aaatagcatt atgtgctgct gaactaacct atttatgttg gatgattaca
3541 cataacggaa cagcaatcaa gagagccaca ttcatgagct ataatactat cataagcaat
3601 tcgctgagtt tggatattgt caacaagtca ctgcagttta aatacaagac gcaaaaagca
3661 acaattctgg aagcctcatt aaagaaattg attcctgctt gggaatttac aattattcct
3721 tactatggac aaaaacatca atctgatatc actgatattg taagtagttt gcaattacag
3781 ttcgaatcat cggaagaagc agataaggga aatagccaca gtaaaaaaat gcttaaagca
3841 cttctaagtg agggtgaaag catctgggag atcactgaga aaatactaaa ttcgtttgag
3901 tatacttcga gatttacaaa aacaaaaact ttataccaat tcctcttcct agctactttc
3961 atcaattgtg gaagattcag cgatattaag aacgttgatc cgaaatcatt taaattagtc
4021 caaaataagt atctgggagt aataatccag tgtttagtga cagagacaaa gacaagcgtt
4081 agtaggcaca tatacttctt tagcgcaagg ggtaggatcg atccacttgt atatttggat
4141 gaatttttga ggaattctga accagtccta aaacgagtaa ataggaccgg caattcttca
4201 agcaacaagc aggaatacca attattaaaa gataacttag tcagatcgta caacaaagct
4261 ttgaagaaaa atgcgcctta ttcaatcttt gctataaaaa atggcccaaa atctcacatt
4321 ggaagacatt tgatgacctc atttctttca atgaagggcc taacggagtt gactaatgtt
4381 gtgggaaatt ggagcgataa gcgtgcttct gccgtggcca ggacaacgta tactcatcag
4441 ataacagcaa tacctgatca ctacttcgca ctagtttctc ggtactatgc atatgatcca
4501 atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga gtggcagcat
4561 atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg gaatgggata
4621 atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat ataatagatt
4681 atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac
4741 aaaacggttt acaagcataa ataaaatggc gccacaggtg cggttgctgg cgcctatatc
4801 gccgacatca ccgatgggag atcctaagat atcgcttagg ccacacgttc aagtgcagcc
4861 acaggataaa tttgcactga gcctgggtgg gattcggact cgaccgcata gccttcagga
4921 gtgagttttg tgcaatacca accgacgact tgaccctgcc aagcggcacc agatttcttg
4981 cgtacgcgat cccctaagcc aaaggtggca ctcaggggaa gcgcaaactg ccctgcaacg
5041 ggagcgttgg cttcatcgct actttgaccc atgtcgaatc cttcttgtga atctattatg
5101 gcgacaagca aatcgagctc tgactgccta ccccacaaca actactagaa agcaccagca
5161 caacggctgc ctaactttgt tttagggcga ctaccctgct gcgtaacatc gttgctgttc
5221 cataacatca aacatcgacc cacggcgtaa cgcgcttagc tagcttggat gcccgaggct
5281 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
5341 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
5401 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcg agctcatcga tatgcattta
5461 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
5521 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
5581 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg
5641 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga
5701 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
5761 aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
5821 atggcgattc agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa
5881 cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
5941 ccccatgcta cctaagcgcc agtgtggttg ccctgcgctg gcgttaaatc ccggaatcgc
6001 cccctgccag tcaagattca gcttcagacg ctccgggcaa taaataatat tctgcaaaac
6061 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc
6121 acgggtaatg cgataagggc gatcgttgag tacatgcagg ccattaccgc gccagacaat
6181 caccagctca caaaaatcat gtgtatgttc agcaaagaca tcttgcggat aacggtcagc
6241 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
6301 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
6361 tacgttgaga agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg
6421 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac
6481 agcgataggc gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca
6541 tcagtcgcag gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
6601 gatgtagcgt acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
6661 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
6721 ggttctcctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc
6781 gactggcggt cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct
6841 gtcgcacctg ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca
6901 gctcttgtgg cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat
6961 acagcacatt ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc
7021 gtacgaaaca gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac
7081 ccgtgccatg ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg
7141 cctgcgggag ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac
7201 tatgtaatac ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga
7261 ggtcaggttc ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa
7321 gatacagcgt gaattttcag gaaatgcggt gagcatcaca tcaccacaat tcagcaaatt
7381 gtgaacatca tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac
7441 gagaaggtcg cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac
7501 attatggata ttaatactga aactgagatc aagcaaaagc attcactaac cccctttcct
7561 gttttcctaa tcagcccggc atttcgcggg cgatattttc acagctattt caggagttca
7621 gccatgaacg cttattacat tcaggatcgt cttgaggctc agagctgggc gcgtcactac
7681 cagcagctcg cccgtgaaga gaaagaggca gaactggcag acgacatgga aaaaggcctg
7741 ccccagcacc tgtttgaatc gctatgcatc gatcatttgc aacgccacgg ggccagcaaa
7801 aaatccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat ggcagaacac
7861 atccggtaca tggttgaaac cattgctcac caccaggttg atattgattc agaggtataa
7921 aacgaatgag tactgcactc gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg
7981 attctgtcga cccacaggaa ctgatcacca ctcttcgcca gacggcattt aaaggtgatg
8041 ccagcgatgc gcagttcatc gcattactga tcgttgccaa ccagtacggc cttaatccgt
8101 ggacgaaaga aatttacgcc tttcctgata agcagaatgg catcgttccg gtggtgggcg
8161 ttgatggctg gtcccgcatc atcaatgaaa accagcagtt tgatggcatg gactttgagc
8221 aggacaatga atcctgtaca tgccggattt accgcaagga ccgtaatcat ccgatctgcg
8281 ttaccgaatg gatggatgaa tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa
8341 tcacggggcc gtggcagtcg catcccaaac ggatgttacg tcataaagcc atgattcagt
8401 gtgcccgtct ggccttcgga tttgctggta tctatgacaa ggatgaagcc gagcgcattg
8461 tcgaaaatac tgcatacact gcagaacgtc agccggaacg cgacatcact ccggttaacg
8521 atgaaaccat gcaggagatt aacactctgc tgatcgccct ggataaaaca tgggatgacg
8581 acttattgcc gctctgttcc cagatatttc gccgcgacat tcgtgcatcg tcagaactga
8641 cacaggccga agcagtaaaa gctcttggat tcctgaaaca gaaagccgca gagcagaagg
8701 tggcagcatg acaccggaca ttatcctgca gcgtaccggg atcgatgtga gagctgtcga
8761 acagggggat gatgcgtggc acaaattacg gctcggcgtc atcaccgctt cagaagttca
8821 caacgtgata gcaaaacccc gctccggaaa gaagtggcct gacatgaaaa tgtcctactt
8881 ccacaccctg cttgctgagg tttgcaccgg tgtggctccg gaagttaacg ctaaagcact
8941 ggcctgggga aaacagtacg agaacgacgc cagaaccctg tttgaattca cttccggcgt
9001 gaatgttact gaatccccga tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc
9061 cgatggttta tgcagtgacg gcaacggcct tgaactgaaa tgcccgttta cctcccggga
9121 tttcatgaag ttccggctcg gtggtttcga ggccataaag tcagcttaca tggcccaggt
9181 gcagtacagc atgtgggtga cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg
9241 tatgaagcgt gaaggcctgc attatgtcgt gattgagcgg gatgaaaagt acatggcgag
9301 ttttgacgag atcgtgccgg agttcatcga aaaaatggac gaggcactgg ctgaaattgg
9361 ttttgtattt ggggagcaat ggcgatagat cctagcaagg aggaaatcat catggctatc
9421 gacgaaaaca aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt
9481 aaaggctcca tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc
9541 ggttcgcttt cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc
9601 gaaatctacg gaccggaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca
9661 gcgcagcgtg aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc
9721 tacgcacgta aactgggcgt cgatatcgac aacctgctgt gctcccagcc ggacaccggc
9781 gagcaggcac tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc
9841 gttgactccg tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct
9901 cacatgggcc ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg
9961 aagcagtcca acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg
10021 ttcggtaacc cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt
10081 ctcgacatcc gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc
10141 cgcgtgaaag tggtgaagaa caaaatcgct gcgccgttta aacaggctga attccagatc
10201 ctctacggcg aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag
10261 ctgatcgaga aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa
10321 gcgaatgcga ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa
10381 gtacgtgagt tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgatagc
10441 gaaggcgtag cagaaactaa cgaagatttt taagctagga tctcgagaag cttgacctgt
10501 gaagtgaaaa atggcgcaca ttgtgcgaca ttttttttgt ctgccgttta ccgctactgc
10561 gtcacggatc tccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
10621 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
10681 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
10741 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
10801 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
10861 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
10921 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
10981 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc aggtggcact
11041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
11101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
//