LOCUS Exported 4506 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4506
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(133..144)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
CDS 756..1454
/codon_start=1
/note="RepA"
/translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
VLDAETGEIK"
CDS complement(1803..2126)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
primer_bind 2036..2052
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2053..2109
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(2122..2138)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2146..2162
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2170..2200)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2215..2236
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2524..3112)
/direction=LEFT
/note="ColE1 ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 3730..4404
/codon_start=1
/note="Spe"
/translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
KL"
ORIGIN
1 actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt
61 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa
121 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat
181 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct
241 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt
301 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct
361 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt
421 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac
481 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag
541 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata
601 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg
661 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag
721 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta
781 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag
841 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata
901 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact
961 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta
1021 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt
1081 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg
1141 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg
1201 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg
1261 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa
1321 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg
1381 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg
1441 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa
1501 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag
1561 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa
1621 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt
1681 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
1741 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
1801 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
1861 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
1921 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
1981 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
2041 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
2101 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
2161 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
2221 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
2281 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
2701 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
2941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
3361 cgtgtagata actagtgttc gtgaatacat gttataataa ctataactaa taacgtaacg
3421 tgactggcaa gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg
3481 aaatgaaaga tcatatcata tataatctag aataaaatta actaaaataa ttattatcta
3541 gataaaaaat ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta
3601 acaactatgg atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca
3661 tacgaacaaa ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt
3721 ggtacttaca tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac
3781 tttttagtcg tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa
3841 attagaccta tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca
3901 attattattc agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat
3961 ggagaatggt tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca
4021 gatttaacca taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat
4081 gacttagagg aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat
4141 tcgtcagagg aattaataga taattatcag gatgatgaaa ccaactctat attaacttta
4201 tgccgtatga ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat
4261 gcagtggctg aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt
4321 tatcttggag agaatattga atggactaat gaaaatgtaa atttaactat aaactattta
4381 aataacagat taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat
4441 ttttttgttt tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta
4501 gaaaac
//