LOCUS Exported 4563 bp ds-DNA circular SYN 26-APR-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pttq18
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4563)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 28, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4563
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 897..1485
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 1927..1955
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 1963..1979
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 1999..2322
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLAAH
PPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSLFWRMREDFQP
DTD"
misc_feature 2001..2057
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(2061..2077)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 2478..2564
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2656..2683
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 3051..3128
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 3129..4211
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 4324..4428
/gene="bla"
/label=AmpR promoter
CDS 4429..726
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
61 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
121 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
181 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
241 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
301 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
361 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
421 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
481 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
541 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
601 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
661 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
721 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
781 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
841 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
1021 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
1081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
1141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
1201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
1261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
1321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
1381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
1441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
1501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
1561 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
1621 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
1681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gaattaattc tcatgtttga
1741 cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc
1801 tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc
1861 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat
1921 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
1981 ttcacacagg aaacagcgat gaattcgagc tcggtacccg gggatcctct agagtcgacc
2041 tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
2101 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
2161 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
2221 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccctg
2281 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
2341 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
2401 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
2461 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
2521 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
2581 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
2641 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
2701 ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag cctcgttttt
2761 gcatcaggaa agcagggaat ttatggtgca ctctcagtac aatctgctct gatgccgcat
2821 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
2881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
2941 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
3001 aggttaatgt catgataata atggtttctt agacgtgagg ttctgtaccc gacaccatcg
3061 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
3121 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
3181 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
3241 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
3301 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
3361 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
3421 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
3481 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
3541 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
3601 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
3661 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
3721 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
3781 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
3841 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
3901 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
3961 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
4021 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
4081 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
4141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
4201 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accccaggct
4261 ttacacttta tgcttccgac ctgcaagaac ctcacgtcag gtggcacttt tcggggaaat
4321 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
4381 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
4441 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
4501 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
4561 atc
//