LOCUS Exported 6034 bp ds-DNA circular SYN 21-NOV-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6034
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 4..1674
/label=CAG promoter
misc_feature 1766..2339
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2372..2971
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_site 3122..3177
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
promoter 3710..3905
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3756..3891
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal 3911..4045
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4284..4872)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5043..5903)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5904..6008)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg
1741 taccgcgggc ccgggatcca ccggtcccct ctccctcccc cccccctaac gttactggcc
1801 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
1861 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
1921 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
1981 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
2041 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
2101 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
2161 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
2221 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
2281 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag
2341 cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct
2401 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
2461 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
2521 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
2581 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
2641 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
2701 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
2761 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
2821 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
2881 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
2941 ctggtgcatg acccgcaagc ccggtgccta ggcggccgca ctcctcaggt gcaggctgcc
3001 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
3061 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
3121 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
3181 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
3241 ttggcaacat atgcccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc
3301 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
3361 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
3421 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
3481 tccctcttct cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
3541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
3601 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
3661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
3721 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3781 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3841 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
3901 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
3961 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
4021 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
4081 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
4141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
4201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
4261 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
4321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
4381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
4441 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
4501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
4561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
4621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
4681 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
4741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
4801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
4861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
4921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
4981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
5041 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
5101 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
5161 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
5221 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
5281 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
5341 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
5401 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
5461 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
5521 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
5581 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
5641 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
5701 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
5761 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
5821 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
5881 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
5941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
6001 gttccgcgca catttccccg aaaagtgcca cctg
//