LOCUS Exported 5769 bp ds-DNA circular SYN 21-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5769
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 475..1653
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
promoter 1670..1688
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1807..1848
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 1858..1875
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 1904..2128
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2174..2602
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2616..2946
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2797..2932
/label=SV40 ori
/note="SV40 origin of replication"
promoter 2994..3041
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 3060..3434
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=Zeo
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 3564..3685
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4136..4724)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4895..5755)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5756..91)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgcctc gtgaggctcc
481 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
541 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
601 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
661 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
721 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
781 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
841 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
901 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
961 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
1021 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
1081 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
1141 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
1201 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
1261 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
1321 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
1381 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
1441 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
1501 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
1561 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
1621 caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact aatacgactc
1681 actataggga gacccaagct ggctagttaa gcttggtacc gagctcggat ccactagtcc
1741 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
1801 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
1861 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
1921 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
1981 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
2041 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
2101 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
2161 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
2221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
2281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
2341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
2401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
2461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
2521 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
2581 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
2641 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2701 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2761 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
2821 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
2881 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
2941 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
3001 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
3061 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
3121 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
3181 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
3241 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
3301 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
3361 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
3421 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
3481 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
3541 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
3601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
3661 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
3721 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
3781 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
3841 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
3901 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
3961 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
4021 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
4081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
4141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
4201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
4261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
4321 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
4381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
4441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
4501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
4561 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
4621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
4681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
4741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
4801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
4861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
4921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
4981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
5041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
5101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
5161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
5221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
5281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
5341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
5401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
5461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
5521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
5581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
5641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
5701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
5761 tcctttttc
//