LOCUS Exported 3861 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3861)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3861
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 546..562
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 602..697
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL4"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind 1906..2030
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(2103..2121)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2126..2142)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 2255..3064
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3211..3799
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aaacggaaga
121 gcgcccaata cgcaaaccgc cctctccccg cgcgttggcc gattcattaa atgcagctgg
181 cacgacaggt tttcccgact ggaaagcggg cagtggagcg caacgcaatt aatacccgta
241 cccgcttacc caggaaaagt ttgtaaaaac gcaaaaaagg ccatccctcc aggatggcct
301 tctgcttaat ttggatgcct ggcaagttta tggggggggt ctgcccgcca ccctccgggc
361 cgttgcttcc acaacgttca aatccgctcc ccgcggattt gtcctactca ggagagcgtt
421 caccgacaaa caacagataa aacgaaaggc ccagttcccg actgagcctt tcgttttatt
481 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
541 acgttgtaaa acgacggcca gtcttaagct cgggcccgcg ttaacgctac catggagctc
601 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
661 tgctttttta taatgccaac tttgtataga aaagttgggc ctcgcgccgg gttttggcgc
721 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga
781 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
841 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
901 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
961 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
1021 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
1081 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
1141 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
1201 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
1261 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
1321 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
1381 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
1441 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac
1501 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
1561 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
1621 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
1681 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
1741 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
1801 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
1861 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagaccaag tttgtacaaa
1921 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
1981 aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg aatcaactac
2041 ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg gccgcgatat
2101 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
2161 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
2221 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
2281 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
2341 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
2401 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
2461 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
2521 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
2581 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
2641 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
2701 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
2761 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
2821 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
2881 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
2941 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
3001 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
3061 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
3121 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
3181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtactctg
3241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
3301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
3361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
3421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
3481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
3541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
3601 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
3661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
3721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
3781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
3841 ctggcctttt gctcacatgt t
//