LOCUS Exported 17251 bp ds-DNA circular SYN 26-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..17251
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 1245..1278
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
protein_bind complement(1302..1335)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 1502..1636
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 1756..1890
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(1919..1952)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 2033..3463
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
misc_feature 3529..4117
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 4144..4351
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 4359..4392
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="minimal attB site for the phi-C31 integrase (Groth
et al., 2000)"
promoter 4414..4913
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
protein_bind 4933..4966
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
CDS 4973..5776
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5786..6247
/label=PGK poly(A) signal
/note="mouse phosphoglycerate kinase 1 polyadenylation
signal"
misc_feature 6313..10653
/label=Rosa26 right arm
/note="right homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
promoter 10703..11202
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 11229..11801
/codon_start=1
/gene="Corynebacterium diphtheriae tox"
/product="diphtheria toxin fragment A"
/label=DTA
/translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
NFETRGKRGQDAMYEYMAQACAGNRVRR"
intron 11878..11943
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 12017..12241
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(12744..13332)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(13503..14363)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(14364..14468)
/gene="bla"
/label=AmpR promoter
rep_origin 14495..14950
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
misc_feature 15932..16725
/label=Rosa26 left arm
/note="left homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
misc_feature 16821..1169
/label=CAG promoter
ORIGIN
1 cgccgggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
61 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
121 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
181 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
241 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
301 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
361 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
421 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
481 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
541 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
601 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
661 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
721 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
781 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
841 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
901 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
961 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
1021 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
1081 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
1141 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
1201 ctcatcattt tggcaaagaa ttgatttgat accgcgggcc ctaagaagtt cctattctct
1261 agaaagtata ggaacttcgt cgacatttaa atcatttaaa tataacttcg tataatgtat
1321 gctatacgaa gttattcgcg atgaataaat gaaagcttgc agatctgcga ctctagagga
1381 tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
1441 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
1501 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
1561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
1621 ttatcatgtc tggatctgcg actctagagg atcataatca gccataccac atttgtagag
1681 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
1741 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
1801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
1861 ctcatcaatg tatcttatca tgtctggatc cccatcaagc tgatccggaa cccttaatat
1921 aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca gcccaagcta
1981 gatcgaattc ggccggcctt gtacgcgtta agtgcaacac gatcccgcca ccatggtgag
2041 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
2101 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
2161 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
2221 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
2281 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
2341 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
2401 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
2461 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
2521 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
2581 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
2641 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
2701 cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc
2761 cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
2821 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
2881 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
2941 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
3001 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
3061 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
3121 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
3181 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
3241 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
3301 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
3361 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
3421 ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagaattgt gttgcactta
3481 acgcgtacaa ggccggccct gcaggaattc gatatcaagc ttatcgataa tcaacctctg
3541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
3601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
3661 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
3721 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
3781 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
3841 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
3901 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
3961 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
4021 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
4081 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgacct
4141 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
4201 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
4261 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
4321 attgggaaga caatggcagg catgctgggg aactagtggt gccagggcgt gcccttgggc
4381 tccccgggcg cggcggccgc atcgaattct accgggtagg ggaggcgctt ttcccaaggc
4441 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
4501 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
4561 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
4621 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
4681 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
4741 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
4801 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
4861 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
4921 atcgccgcta gcgaagttcc tattctctag aaagtatagg aacttcgcca ccatgggatc
4981 ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
5041 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
5101 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
5161 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
5221 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
5281 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
5341 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
5401 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
5461 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
5521 cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
5581 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
5641 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
5701 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
5761 tgacgagttc ttctgagggg atccgctgta agtctgcaga aattgatgat ctattaaaca
5821 ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa
5881 cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat
5941 tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat
6001 agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac
6061 tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt gattcccact
6121 ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat
6181 cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc
6241 atcagaaagc ttgcagaaga tctccccaac tggggtaacc tttgagttct ctcagttggg
6301 ggggcgcgcc ggctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg
6361 tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc
6421 ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaataagg aaaatgggag
6481 gataggtagt catctggggt tttatgcagc aaaactacag gttattattg cttgtgatcc
6541 gcctcggagt attttccatc gaggtagatt aaagacatgc tcacccgagt tttatactct
6601 cctgcttgag atccttacta cagtatgaaa ttacagtgtc gcgagttaga ctatgtaagc
6661 agaattttaa tcatttttaa agagcccagt acttcatatc catttctccc gctccttctg
6721 cagccttatc aaaaggtatt ttagaacact cattttagcc ccattttcat ttattatact
6781 ggcttatcca acccctagac agagcattgg cattttccct ttcctgatct tagaagtctg
6841 atgactcatg aaaccagaca gattagttac atacaccaca aatcgaggct gtagctgggg
6901 cctcaacact gcagttcttt tataactcct tagtacactt tttgttgatc ctttgccttg
6961 atccttaatt ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca tttgggccta
7021 ttctcagtcc agggagtttt acaacaatag atgtattgag aatccaacct aaagcttaac
7081 tttccactcc catgaatgcc tctctccttt ttctccattt ataaactgag ctattaacca
7141 ttaatggttt ccaggtggat gtctcctccc ccaatattac ctgatgtatc ttacatattg
7201 ccaggctgat attttaagac attaaaaggt atatttcatt attgagccac atggtattga
7261 ttactgctta ctaaaatttt gtcattgtac acatctgtaa aaggtggttc cttttggaat
7321 gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt gtattttttc
7381 tatttaagca gtgctttctc ttggactggc ttgactcatg gcattctaca cgttattgct
7441 ggtctaaatg tgattttgcc aagcttcttc aggacctata attttgcttg acttgtagcc
7501 aaacacaagt aaaatgatta agcaacaaat gtatttgtga agcttggttt ttaggttgtt
7561 gtgttgtgtg tgcttgtgct ctataataat actatccagg ggctggagag gtggctcgga
7621 gttcaagagc acagactgct cttccagaag tcctgagttc aattcccagc aaccacatgg
7681 tggctcacaa ccatctgtaa tgggatctga tgccctcttc tggtgtgtct gaagaccaca
7741 agtgtattca cattaaataa ataaatcctc cttcttcttc tttttttttt ttttaaagag
7801 aatactgtct ccagtagaat ttactgaagt aatgaaatac tttgtgtttg ttccaatatg
7861 gtagccaata atcaaattac tctttaagca ctggaaatgt taccaaggaa ctaattttta
7921 tttgaagtgt aactgtggac agaggagcca taactgcaga cttgtgggat acagaagacc
7981 aatgcagact ttaatgtctt ttctcttaca ctaagcaata aagaaataaa aattgaactt
8041 ctagtatcct atttgtttaa actgctagct ttacttaact tttgtgcttc atctatacaa
8101 agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga taattttgga
8161 tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct ttatgccttt
8221 aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc agcctggtct
8281 acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt tggggagggg
8341 ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac tatggatgtg
8401 gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc tctgctgttg
8461 gttgggtctt ttgacactgt gggctttctt taaagcctcc ttcctgccat gtggtctctt
8521 gtttgctact aacttcccat ggcttaaatg gcatggcttt ttgccttcta agggcagctg
8581 ctgagatttg cagcctgatt tccagggtgg ggttgggaaa tctttcaaac actaaaattg
8641 tcctttaatt ttttttttaa aaaatgggtt atataataaa cctcataaaa tagttatgag
8701 gagtgaggtg gactaatatt aaatgagtcc ctcccctata aaagagctat taaggctttt
8761 tgtcttatac ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg tcttagagtt
8821 ttagtataca gaaactgttg catcgcttaa tcagattttc tagtttcaaa tccagagaat
8881 ccaaattctt cacagccaaa gtcaaattaa gaatttctga cttttaatgt taatttgctt
8941 actgtgaata taaaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct
9001 gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg
9061 ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta
9121 agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt gaattattat
9181 caccgcaacc tactttttaa aaaaaaaagc caggcctgtt agagcatgct taagggatcc
9241 ctaggacttg ctgagcacac aagagtagtt acttggcagg ctcctggtga gagcatattt
9301 caaaaaacaa ggcagacaac caagaaacta cagttaaggt tacctgtctt taaaccatct
9361 gcatatacac agggatatta aaatattcca aataatattt cattcaagtt ttcccccatc
9421 aaattgggac atggatttct ccggtgaata ggcagagttg gaaactaaac aaatgttggt
9481 tttgtgattt gtgaaattgt tttcaagtga tagttaaagc ccatgagata cagaacaaag
9541 ctgctatttc gaggtctctt ggtttatact cagaagcact tctttgggtt tccctgcact
9601 atcctgatca tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa cttaagtttc
9661 ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa acatgttata tatgttaaac
9721 atttgtactt aatgtgaaag ttaggtcttt gtgggtttga tttttaattt tcaaaacctg
9781 agctaaataa gtcattttta catgtcttac atttggtgga attgtataat tgtggtttgc
9841 aggcaagact ctctgaccta gtaaccctac ctatagagca ctttgctggg tcacaagtct
9901 aggagtcaag catttcacct tgaagttgag acgttttgtt agtgtatact agtttatatg
9961 ttggaggaca tgtttatcca gaagatattc aggactattt ttgactgggc taaggaattg
10021 attctgatta gcactgttag tgagcattga gtggccttta ggcttgaatt ggagtcactt
10081 gtatatctca aataatgctg gcctttttta aaaagccctt gttctttatc accctgtttt
10141 ctacataatt tttgttcaaa gaaatacttg tttggatctc cttttgacaa caatagcatg
10201 ttttcaagcc atattttttt tccttttttt tttttttttt ggtttttcga gacagggttt
10261 ctctgtatag ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca
10321 gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc accacgcctg
10381 gctaagttgg atattttgtt atataactat aaccaatact aactccactg ggtggatttt
10441 taattcagtc agtagtctta agtggtcttt attggccctt cattaaaatc tactgttcac
10501 tctaacagag gctgttggta ctagtggcac ttaagcaact tcctacggat atactagcag
10561 attaagggtc agggatagaa actagtctag cgttttgtat acctaccagc tttatactac
10621 cttgttctga tagaaatatt tcaggacatc tagcttatcg ataccgtcga cggtatcgat
10681 aagcttgata tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
10741 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
10801 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
10861 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
10921 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
10981 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
11041 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
11101 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
11161 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag gtcctcgcca
11221 tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac ttttcttcgt
11281 accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa aagccaaaat
11341 ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc gacaataaat
11401 acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa gctggaggcg
11461 tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg gataatgccg
11521 aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag caagtcggaa
11581 cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc agccttccct
11641 tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg aaagcgttaa
11701 gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat gcgatgtatg
11761 agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt gaaggaacct
11821 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
11881 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
11941 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga tcctagagct
12001 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
12061 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
12121 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
12181 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
12241 gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc cagcttttgt
12301 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
12361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
12421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
12481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
12541 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
12601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
12661 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
12721 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
12781 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
12841 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
12901 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
12961 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
13021 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
13081 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
13141 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
13201 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
13261 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
13321 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
13381 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
13441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
13501 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
13561 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
13621 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
13681 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
13741 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
13801 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
13861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
13921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
13981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
14041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
14101 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
14161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
14221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
14281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
14341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
14401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
14461 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
14521 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
14581 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
14641 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
14701 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
14761 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
14821 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
14881 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
14941 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
15001 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
15061 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
15121 acgactcact atagggcgaa ttggagctcc accgcggcag gccctccgag cgtggtggag
15181 ccgttctgtg agacagccgg gtacgagtcg tgacgctgga aggggcaagc gggtggtggg
15241 caggaatgcg gtccgccctg cagcaaccgg agggggaggg agaagggagc ggaaaagtct
15301 ccaccggacg cggccatggc tcgggggggg ggggggggca gcggaggagc gcttccggcc
15361 gacgtctcgt cgctgattgg cttcttttcc tcccgccgtg tgtgaaaaca caaatggcgt
15421 gttttggttg gcgtaaggcg cctgtcagtt aacggcagcc ggagtgcgca gccgccggca
15481 gcctcgctct gcccactggg tggggcggga ggtaggtggg gtgaggcgag ctggacgtgc
15541 gggcgcggtc ggcctctggc ggggcggggg gggggggggg gcagcggagg agcgcttccg
15601 gccgacgtct cgtcgctgat tggcttcttt tcctcccgcc gtgtgtgaaa acacaaatgg
15661 cgtgttttgg ttggcgtaag gcgcctgtca gttaacggca gccggagtgc gcagccgccg
15721 gcagcctcgc tctgcccact gggtggggcg ggaggtaggt ggggtgaggc gagctggacg
15781 tgcgggcgcg gtcggcctct ggcggggcgg gggggggggg ggggcagcgg aggagcgctt
15841 ccggccgacg tctcgtcgct gattggcttc ttttcctccc gccgtgtgtg aaaacacaaa
15901 tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg gcagccggag tgcgcagccg
15961 ccggcagcct cgctctgccc actgggtggg gcgggaggta ggtggggtga ggcgagctgg
16021 acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg gagggagggt cagcgaaagt
16081 agctcgcgcg cgagcggccg cccaccctcc ccttcctctg ggggagtcgt tttacccgcc
16141 gccggccggg cctcgtcgtc tgattggctc tcggggccca gaaaactggc ccttgccatt
16201 ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc gggggcggcg aggaggcgct
16261 cccaggttcc ggccctcccc tcggccccgc gccgcagagt ctggccgcgc gcccctgcgc
16321 aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag tagggctgag cggctgcggg
16381 gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag gggcgtgctg agccagacct
16441 ccatcgcgca ctccggggag tggagggaag gagcgagggc tcagttgggc tgttttggag
16501 gcaggaagca cttgctctcc caaagtcgct ctgagttgtt atcagtaagg gagctgcagt
16561 ggagtaggcg gggagaaggc cgcacccttc tccggagggg ggaggggagt gttgcaatac
16621 ctttctggga gttctctgct gcctcctggc ttctgaggac cgccctgggc ctgggagaat
16681 cccttccccc tcttccctcg tgatctgcaa ctccagtctt tctagcctta attaaccgtt
16741 taaacaattc tgcaggaatc tagttattaa tagtaatcaa ttacggggtc attagttcat
16801 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
16861 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
16921 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
16981 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
17041 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
17101 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
17161 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
17221 gcgatggggg cggggggggg gggggggcgc g
//