LOCUS Exported 6139 bp ds-DNA circular SYN 19-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6139
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 202..581
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 582..785
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 830..848
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 890..899
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 896..1606
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
polyA_signal 1706..1930
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1976..2404
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2418..2747
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2598..2733
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2814..3608
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3782..3903
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4354..4939)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5110..5970)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5971..6075)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatggt
901 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
961 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
1021 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
1081 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
1141 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
1201 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
1261 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
1321 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
1381 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
1441 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
1501 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
1561 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtagggat ccactagtcc
1621 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
1681 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
1741 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
1801 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
1861 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
1921 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
1981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
2041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
2101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
2161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
2221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
2281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
2341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
2401 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
2461 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
2521 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
2581 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
2641 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
2701 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
2761 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
2821 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
2881 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
2941 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
3001 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
3061 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
3121 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
3181 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
3241 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
3301 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
3361 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
3421 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
3481 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
3541 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
3601 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
3661 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
3721 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
3781 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3841 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3901 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
3961 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
4021 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
4081 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
4141 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
4201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
4261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
4321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
4801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4861 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
4921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
4981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
5041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
5101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
5161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
5221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
5281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
5341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
5401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
5461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
5521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
5581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
5641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
5701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
5761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
5821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
5881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
5941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
6001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
6061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
6121 tctcccgatc ccctatggt
//