LOCUS Exported 4729 bp ds-DNA circular SYN 18-10-2015
FEATURES Location/Qualifiers
source 1..4729
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 609..665
/note="MCS"
/note="multiple cloning site"
CDS 675..1394
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 1517..1638
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1645..2100)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2127..2231
/gene="bla"
/note="AmpR promoter"
promoter 2233..2590
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2441..2576
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2625..3419
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3651..3698
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4027..4615
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccatcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
1021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
1081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
1141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
1201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
1261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
1321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
1381 agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
1441 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
1501 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
1561 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
1621 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
1681 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
1741 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
1801 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
1861 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
1921 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
1981 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
2041 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
2101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
2161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
2221 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
2281 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
2341 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
2401 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
2461 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
2521 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
2581 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
2641 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
2701 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
2761 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
2821 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
2881 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
2941 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
3001 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
3061 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
3121 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
3181 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
3241 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
3301 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
3361 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
3421 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
3481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
3541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
3601 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
3661 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
3721 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
3781 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
3841 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
3901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
3961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
4021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
4081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
4141 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
4441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
4501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
4561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
4621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
4681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//