LOCUS Exported 4732 bp ds-DNA circular SYN 26-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4732)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-18 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4732
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="CMV enhancer;human cytomegalovirus immediate early
enhancer"
promoter 365..568
/label=CMV promoter
/note="CMV promoter;human cytomegalovirus (CMV) immediate
early promoter"
misc_feature 591..671
/label=MCS
/note="MCS;multiple cloning site"
CDS 679..1398
/codon_start=1
/product="yellow fluorescent protein with a large Stokes
shift, excitable with violet light (Ai et al., 2008)"
/label=mAmetrine
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
EFVTAAGITPGMDELYK"
polyA_signal 1520..1641
/label=SV40 poly(A) signal
/note="SV40 poly(A) signal;SV40 polyadenylation signal"
rep_origin complement(1648..2103)
/direction=LEFT
/label=f1 ori
/note="f1 ori;f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2130..2234
/gene="bla"
/label=AmpR promoter
/note="AmpR promoter"
promoter 2236..2593
/label=SV40 promoter
/note="SV40 promoter;SV40 enhancer and early promoter"
rep_origin 2444..2579
/label=SV40 ori
/note="SV40 ori;SV40 origin of replication"
CDS 2628..3422
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="NeoR/KanR;confers resistance to neomycin, kanamycin,
and G418 (Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3654..3701
/label=HSV TK poly(A) signal
/note="HSV TK poly(A) signal;herpesvirus thymidine kinase
polyadenylation signal"
rep_origin 4030..4618
/direction=RIGHT
/label=ori
/note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc
781 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcacctc cggcaagctg
841 cccgtgccct ggcccaccct cgtgaccacc ctgtcttacg gcgtgcagtg cttcgcccgc
901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
961 caggagcgca ccatctcctt caaggacgac ggcagctaca ggacccgcgc cgaggtgaag
1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
1081 ggcaacatcc tggggcacaa gctggagtac aacatgaacg tgtgggacgc gtatatcacg
1141 gccgacaagc agaagaacgg catcaaagcg aacttcaaga tcgagcacaa cgtcgaggac
1201 ggcggcgtgc agctcgccga cgcgtaccag cagaacaccc ccatcggcga cggctccgtg
1261 ctgctgcctg acaaccacta cctgagcttc cagagcaagc tgttcaaaga ccccaacgag
1321 cagcgcgatc acatggtcct gctggagttc gttaccgccg ccgggatcac tcccggcatg
1381 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
1441 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
1501 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
1561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
1621 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
1681 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
1741 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
1801 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
1861 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
1921 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
1981 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
2041 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
2101 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
2161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
2221 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
2281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
2461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
2521 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
2581 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
2641 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
2701 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
2761 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
2821 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
2881 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
2941 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
3001 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
3061 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
3121 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
3181 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
3241 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
3301 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
3361 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
3421 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
3481 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
3541 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
3601 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
3661 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
3721 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
3781 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
3841 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
3901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
3961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
4021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
4081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
4141 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
4201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
4261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
4321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
4381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
4441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
4501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
4561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
4621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
4681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
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