LOCUS Exported 5442 bp ds-DNA circular SYN 01-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5442)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5442
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..2040
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/note="tdTomato"
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
misc_feature 2041..2106
/note="MCS"
/note="multiple cloning site"
polyA_signal 2230..2351
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2358..2813)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2840..2944
/gene="bla"
/note="AmpR promoter"
promoter 2946..3303
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3154..3289
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3338..4132
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4364..4411
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4740..5328
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
661 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
721 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
781 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
841 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
901 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
961 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
1021 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
1081 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
1141 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
1201 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
1261 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
1321 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
1381 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
1441 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
1501 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
1561 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
1621 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
1681 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
1741 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
1801 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
1861 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
1921 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
1981 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
2041 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
2101 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
2161 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
2221 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2341 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
2401 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
2461 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
2521 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
2581 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
2641 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
2701 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
2761 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
2821 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
2881 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
2941 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
3001 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
3061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
3121 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
3181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
3241 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
3301 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
3361 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
3421 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
3481 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
3541 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
3601 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
3661 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
3721 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
3781 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
3841 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
3901 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
3961 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
4021 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
4081 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
4141 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
4201 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
4261 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
4321 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
4381 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
4441 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
4501 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
4561 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
4621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
4681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
4741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
4801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
4861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
4921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
4981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
5101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
5161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
5221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
5281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
5341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
5401 ttatcccctg attctgtgga taaccgtatt accgccatgc at
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