LOCUS Exported 4880 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4880)
AUTHORS .
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4880
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 37..817
/label=CHOP promoter (-649/+136)
regulatory 831..840
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 837..1547
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
polyA_signal 1668..1789
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1796..2251)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2278..2382
/gene="bla"
/label=AmpR promoter
promoter 2384..2741
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2592..2727
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2776..3570
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3802..3849
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4178..4766
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattac tagcgctacc ggactcagat ctcgagatgt cgacaatccc agtggatgga
61 taccaacttt aaaaagaaaa gttcaaaagg cctatgtgcc cattagctgg gaggggccaa
121 gaaatatggg agtcccttat agtgggggta aaacggcggg taaagctagg tgggcggaac
181 agcagcttct gggggagaca agcggcaaag aggctcacga ccgactaggg gcgaccaagg
241 ctgatagccg ttggggccgt tgggcgccgg gagctggcgc cccgccctct ctcctctccc
301 ccaccctccg cacctcccac caccctcggt gtcccctgcg cgtgcgcgtg cagacaccgg
361 ttgccaaaca ttgcatcatc cccgcccccc tttcctcccc tccccccccg ctacactccc
421 ctccgcgcgc gcgcatgact cacccacctc ctccgtgaag cctcgtgacc caaagccact
481 tccgggtccg acactacgtc gaccccctag cgagagggag cgacgggggc ggtgccgcgg
541 ggctcctgag tggcggatgc gagggacggg gcgggaccaa tgccggcgtg ccactttctg
601 attggtaggt tttggggtcc cgcccctgag aggagggcaa ggccatggta aaagattaca
661 gccaggcgct cccgaggtca gagacttaag tctaaggcac tgagcgtatc atgttaaaga
721 tgagcgggtg gcagcgacag agccaaaatc agagctggaa cctgaggaga gagtgttcaa
781 gaaggaagtg tatcttcata catcaccaca cctgacggga tccaccggtc gccaccatgg
841 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
901 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
961 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
1021 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
1081 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
1141 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
1201 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
1261 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
1321 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
1381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
1441 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
1501 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagcgg ccgcgactct
1561 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
1621 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
1681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
1741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
1801 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
1861 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
1921 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
1981 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
2041 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
2101 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
2161 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
2221 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
2401 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
2461 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
2521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
2581 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
2641 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
2701 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
2761 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
2821 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
2881 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
2941 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
3001 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
3061 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
3121 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
3181 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
3241 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
3301 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
3361 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
3421 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
3481 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
3541 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
3601 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
3661 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
3721 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
3781 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
3841 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
3901 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
3961 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
4021 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
4081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
4141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
4201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
4261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
4321 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
4381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
4441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
4501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
4561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
4621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
4681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
4741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
4801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
4861 accgtattac cgccatgcat
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