LOCUS Exported 4698 bp ds-DNA circular SYN 22-AUG-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4698)
TITLE Direct Submission
JOURNAL Exported Aug 25, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4698
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1296
/codon_start=1
/product="far-red fluorescent variant of Heteractis crispa
chromoprotein"
/label=HcRed1
/note="mammalian codon-optimized"
/translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
LPEKAN"
misc_feature 1297..1362
/label=MCS
/note="multiple cloning site"
polyA_signal 1486..1607
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1614..2069)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2096..2200
/gene="bla"
/label=AmpR promoter
promoter 2202..2559
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2410..2545
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2594..3388
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3620..3667
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3996..4584
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag cggcctgctg aaggagagta tgcgcatcaa gatgtacatg
661 gagggcaccg tgaacggcca ctacttcaag tgcgagggcg agggcgacgg caaccccttc
721 gccggcaccc agagcatgag aatccacgtg accgagggcg cccccctgcc cttcgccttc
781 gacatcctgg ccccctgctg cgagtacggc agcaggacct tcgtgcacca caccgccgag
841 atccccgact tcttcaagca gagcttcccc gagggcttca cctgggagag aaccaccacc
901 tacgaggacg gcggcatcct gaccgcccac caggacacca gcctggaggg caactgcctg
961 atctacaagg tgaaggtgca cggcaccaac ttccccgccg acggccccgt gatgaagaac
1021 aagagcggcg gctgggagcc cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc
1081 ggccggaacg tgatggccct gaaggtgggc gaccggcacc tgatctgcca ccactacacc
1141 agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac
1201 atccggctcc agatgctgcg gaagaagaag gacgagtact tcgagctgta cgaggccagc
1261 gtggcccggt acagcgacct gcccgagaag gccaactccg gactcagatc tcgagctcaa
1321 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg
1381 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
1441 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
1621 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
1681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
1741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
1801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
1861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
1921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
1981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
2041 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
2101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
2161 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
2221 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
2281 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
2341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
2401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
2461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
2521 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
2581 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
2641 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
2701 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
2761 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
2821 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
2881 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
2941 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
3001 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
3061 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
3121 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
3181 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
3241 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
3301 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
3361 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
3421 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
3481 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
3541 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
3601 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
3661 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
3721 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
3781 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
3841 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
3901 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
3961 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
4021 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
4081 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
4141 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
4201 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
4261 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
4321 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
4381 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
4441 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
4501 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
4561 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
4621 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
4681 cgtattaccg ccatgcat
//