LOCUS Exported 4150 bp ds-DNA circular SYN 18-10-2015
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4150)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-18 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4150
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 12..89
/note="MCS"
/note="multiple cloning site"
CDS 97..816
/codon_start=1
/product="Aequorea coerulescens GFP"
/note="AcGFP1"
/note="mammalian codon-optimized"
/translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
GFVTAAAITHGMDELYK"
polyA_signal 938..1059
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1066..1521)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1548..1652
/gene="bla"
/note="AmpR promoter"
promoter 1654..2011
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1862..1997
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2046..2840
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3072..3119
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3448..4036
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
61 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgccgagctg
121 ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg ccacaagttc
181 agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
241 tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tgaccaccct gagctacggc
301 gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt caagagcgcc
361 atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg caactacaag
421 tcgcgcgccg aggtgaagtt cgagggcgat accctggtga atcgcatcga gctgaccggc
481 accgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa ctacaacgcc
541 cacaatgtgt acatcatgac cgacaaggcc aagaatggca tcaaggtgaa cttcaagatc
601 cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc actaccagca gaataccccc
661 atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca gagcgccctg
721 tccaaggacc ccaacgagaa gcgcgatcac atgatctact tcggcttcgt gaccgccgcc
781 gccatcaccc acggcatgga tgagctgtac aagtgagcgg ccgcgactct agatcataat
841 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
901 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
1021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
1081 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
1141 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
1201 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
1261 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
1321 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
1381 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
1441 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
1501 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
1561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
1621 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
1681 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
1741 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
1801 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
1861 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
1921 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
1981 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
2041 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
2101 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
2161 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
2221 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
2281 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
2341 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
2401 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
2461 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
2521 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
2581 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
2641 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
2701 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
2761 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
2821 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
2881 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
2941 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
3001 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
3061 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
3121 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
3181 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
3241 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
3301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
3361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
3421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
3481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
3541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
3601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
3661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
3721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
3781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
3841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
3901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
3961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
4021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
4081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
4141 cgccatgcat
//