产品 资讯
请输入产品名称
噪声分析仪 纺织检测仪器 Toc分析仪 PT-303红外测温仪 转矩测试仪 继电保护试验仪 定氮仪
首页 产品 专题 品牌 资料 展会 成功案例 网上展会
词多 效果好 就选易搜宝！
首页 >>> 产品目录 >>> 分子生物学 >>> 质粒（载体）
仪表展览网 >>> 展馆展区 >>> 试剂 >>> **试剂 >>> pDsRed2-C1 > pDsRed2-C1

产品资料

pDsRed2-C1

pDsRed2-C1
  • 如果您对该产品感兴趣的话，可以
  • 产品名称：pDsRed2-C1
  • 产品型号：
  • 产品展商：XYbscience
  • 产品文档：无相关文档
简单介绍
pDsRed2-C1一种DsRed变体，其设计用于更快的成熟和较低的非特异性聚集。衍生自Discosoma sp。红色荧光蛋白DsRed2，如其祖细胞DsRed1，含有一系列沉默的碱基对变化,对应于哺乳动物细胞中高表达的人类密码子使用偏好。pDsRed2-C1除了这些变化外，DsRed2含有六个氨基酸取代：V105A，I161T和S197A，导致转染细胞系中红色荧光的出现更加快速;和R2A，K5E和K9T，其阻止蛋白质聚集。（DsRed2可能与DsRed1形成相同的四聚体结构。）
产品描述

pDsRed2-C1

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0140 pDsRed2-C1

5μg

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: CMV promoter
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳系列质粒；哺乳荧光质粒；哺乳红色质粒
质粒大小: 4675bp
质粒标签: N-DsRed2
原核抗性: 卡那霉素Kan（50μg/ml）
筛选标记: 新霉素Neo/G418
克隆菌株: DH5α等大肠杆菌
培养条件: 37℃，有氧 LB
表达宿主: 293T等哺乳细胞
培养条件: 37℃，5CO2
诱导方式: 无须诱导，瞬时表达
5'测序引物: CMV-F（CGCAAATGGGCGGTAGGCGTG）
3'测序引物: Sv40-polyA-R (GAAATTTGTGATGCTATTGC)
备注: 哺乳细胞红色荧光表达质粒

质粒简介

        pDsRed2-C1编码DsRed2,一种DsRed变体，其设计用于更快的成熟和较低的非特异性聚集。衍生自Discosoma sp。红色荧光蛋白DsRed2，如其祖细胞DsRed1，含有一系列沉默的碱基对变化,对应于哺乳动物细胞中高表达的人类密码子使用偏好。除了这些变化外，DsRed2含有六个氨基酸取代：V105A，I161T和S197A，导致转染细胞系中红色荧光的出现更加快速;和R2A，K5E和K9T，其阻止蛋白质聚集。（DsRed2可能与DsRed1形成相同的四聚体结构。）在DsRed2组成型表达的哺乳动物细胞培养物中，转染后24小时内可通过荧光显微镜检测发红细胞。在表达DsRed1的**和哺乳动物细胞系统中经常观察到的蛋白质的大不溶性聚集体在表达DsRed2的生物体中显着降低。更快成熟，更可溶性的红色荧光蛋白也被宿主细胞耐受良好;用DsRed2转染的哺乳动物细胞培养物在测试的这些细胞系中没有显示出降低的存活力的明显迹象，表达DsRed2的细胞显示与非转染对照相同的形态和生长特征。     
       pDsRed2-C1 encodes DsRed2, a DsRed variant that has been engineered for faster maturation and lower non-specifc aggregation. Derived from the Discosoma sp. red fuorescent protein (drFP583; 1), DsRed2, like its progenitor DsRed1, contains a series of silent base-pair changes that correspond to human codon-usage preferences for high expression in mammalian cells (2). In addition to these changes, DsRed2 contains six amino acid substitutions: V105A, I161T, and S197A, which result in the more rapid appearance of red fuorescence in transfected cell lines; and R2A, K5E, and K9T, which prevent the protein from aggregating. (DsRed2 may, however, form the same tetrameric structure as DsRed1 [3].) In mammalian cell cultures when DsRed2 is expressed constitutively, red-emitting cells can be detected by fuorescence microscopy within 24 hours of transfection. Large insoluble aggregates of protein, often observed in bacterial and mammalian cell systems expressing DsRed1, are dramatically reduced in organisms expressing DsRed2. The faster-maturing, more soluble red fuorescent protein is also well tolerated by host cells; mammalian cell cultures transfected with DsRed2 show no obvious signs of reduced viability—in those cell lines tested, cells expressing DsRed2 display the same morphology (e.g., adherence, light-refraction) and growth characteristics as non-transfected controls.
        The multiple cloning site (MCS) in pDsRed2-C1 is positioned between the DsRed2 coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of DsRed2 if they are in the same reading frame as DsRed2 and there are no intervening stop codons. A Kozak consensus translation initiation site upstream of DsRed2 increases the translation efficiency in eukaryotic cells (4). SV40 poly A signals downstream of the MCS direct proper processing of the 3' end of mRNA transcripts. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette (Neor ), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli.
       pDsRed2-C1 can be used to construct fusions to the C-terminus of DsRed2. If a fusion construct retains the fluorescent properties of the native DsRed2 protein, its expression can be monitored by flow cytometry and its localization in vivo can be determined by fluorescence microscopy. The target gene should be cloned into pDsRed2-C1 so that it is in frame with the DsRed2 coding sequences, with no intervening in-frame stop codons. The recombinant DsRed2 vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (5). pDsRed2-C1 can also be used as a cotransfection marker; the unmodified vector will express DsRed2.

质粒图谱



质粒序列

LOCUS       Exported File           4675 bp ds-DNA    circular SYN 21-4-2015
KEYWORDS    pDsRed2-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4675)
  TITLE     Direct Submission
  JOURNAL   Exported 2015-4-21 from MiaoLingPlasmid
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             613..1287
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /note="DsRed2"
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     misc_feature    1288..1344
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1468..1589
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1596..2051)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2078..2182
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2184..2541
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2392..2527
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2576..3370
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3602..3649
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3978..4566
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg
      661 cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      721 ccctacgagg gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc
      781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc
      841 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      901 atgaacttcg aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc
      961 tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg
     1021 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     1081 ctgaagggcg agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag
     1141 ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac
     1201 gccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc
     1261 accgagggcc gccaccacct gttcctgaga tctcgagctc aagcttcgaa ttctgcagtc
     1321 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     1381 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     1441 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     1501 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1561 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     1621 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     1681 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     1741 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     1801 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     1861 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     1921 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     1981 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2041 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2161 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     2221 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2281 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2341 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2461 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2521 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     2581 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     2641 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     2701 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     2761 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     2821 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     2881 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     2941 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3001 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3061 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     3121 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3181 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3241 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3301 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3361 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     3421 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     3481 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3541 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     3601 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     3661 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     3721 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     3781 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     3841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4621 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcca
//
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
  • 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
  • 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报
产品留言
标题
内容
联系人
联系电话
电子邮件
公司名称
联系地址
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!