LOCUS Exported 4691 bp ds-DNA circular SYN 22-10-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4691)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-22 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4691
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/note="MCS"
/note="multiple cloning site"
CDS 679..1356
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Strongin et al., 2007)"
/note="DsRed-Monomer"
/note="mammalian codon-optimized"
/translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
GSQ"
polyA_signal 1479..1600
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1607..2062)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2089..2193
/gene="bla"
/note="AmpR promoter"
promoter 2195..2552
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2403..2538
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2587..3381
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3613..3660
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3989..4577
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggacaacacc gaggacgtca tcaaggagtt catgcagttc
721 aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg cgagggcgag
781 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg
841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc ctacgtgaag
901 caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt cacctgggag
961 cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc ctccctgcag
1021 gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc cgacggcccc
1081 gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta cccccaggac
1141 ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg ccactacacc
1201 tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg caaccactac
1261 gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt ggagcagtac
1321 gagcacgccg aggcccgcca ctccggctcc cagtagagcg gccgcgactc tagatcataa
1381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
1441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
1561 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
1621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
1681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
1741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
1801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
1861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
1921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
1981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
2041 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
2161 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
2221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
2281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
2401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
2461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
2521 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
2581 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
2641 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
2701 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
2761 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
2821 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
2881 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
2941 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
3001 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
3061 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
3121 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
3181 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
3241 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
3301 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
3361 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
3421 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
3481 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
3541 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
3601 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
3661 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
3721 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
3781 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
3841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
3901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
3961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
4021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
4081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
4141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
4201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
4261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
4321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
4621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
4681 ccgccatgca t
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