LOCUS Exported 4693 bp ds-DNA circular SYN 29-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4693)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 29, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4693
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/note="MCS"
/note="multiple cloning site"
CDS 679..1359
/codon_start=1
/product="monomeric variant of green-to-red photoswitchable
fluorescent protein EosFP (McKinney et al., 2009)"
/note="mEos2"
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
polyA_signal 1481..1602
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1609..2064)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2091..2195
/gene="bla"
/note="AmpR promoter"
promoter 2197..2554
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2405..2540
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2589..3383
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3615..3662
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3991..4579
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg
721 gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt
781 gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt
841 gatatcctga ccactgcatt catgtacggc aacagggtat tcgccaaata tccagacaac
901 atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact
961 ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc
1021 tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag
1081 aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg
1141 ggtgatattc atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga
1201 actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac
1261 tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct
1321 gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatcat
1381 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
1441 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
1501 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
1561 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
1621 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
1681 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
1741 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
1801 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
1861 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
1921 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
1981 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
2041 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2101 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2161 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
2221 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2281 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2341 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2401 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2461 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2521 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
2581 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
2641 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
2701 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
2761 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
2821 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
2881 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
2941 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
3001 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
3061 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
3121 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3181 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3241 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3301 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3361 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3421 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
3481 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
3541 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
3601 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
3661 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
3721 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
3781 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
3841 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
3901 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
3961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
4141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4381 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
4561 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
4621 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
4681 taccgccatg cat
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