LOCUS Exported 4680 bp ds-DNA circular SYN 01-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4680)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4680
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1287
/codon_start=1
/product="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
/note="DsRed-Express"
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
LFL"
misc_feature 1288..1344
/note="MCS"
/note="multiple cloning site"
polyA_signal 1468..1589
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1596..2051)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2078..2182
/gene="bla"
/note="AmpR promoter"
promoter 2184..2541
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2392..2527
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2576..3370
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3602..3649
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3978..4566
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg
661 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
721 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc
841 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
901 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
961 tccttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg
1021 cagaagaaga ctatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
1081 ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag
1141 ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac
1201 tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc
1261 gccgagggcc gccaccacct gttcctgaga tctcgagctc aagcttcgaa ttctgcagtc
1321 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
1381 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
1441 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
1501 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
1561 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
1621 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
1681 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
1741 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
1801 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
1861 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
1921 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
1981 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
2041 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
2161 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
2221 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2281 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2341 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2461 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
2521 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
2581 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
2641 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
2701 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
2761 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
2821 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
2881 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
2941 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
3001 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
3061 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
3121 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
3181 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
3241 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
3301 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
3361 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
3421 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
3481 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
3541 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
3601 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
3661 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
3721 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
3781 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
3841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
3901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
3961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
4021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
4081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
4141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
4201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
4321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
4441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
4501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
4561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
4621 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//