LOCUS Exported 4710 bp ds-DNA circular SYN 29-NOV-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pmScarlet-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4710)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, November 29, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4710
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 613..1300
/note="mScarlet"
misc_feature 1309..1374
/note="MCS"
/note="multiple cloning site"
polyA_signal 1498..1619
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1626..2081)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2108..2212
/gene="bla"
/note="AmpR promoter"
promoter 2214..2571
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2422..2557
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2606..3400
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3632..3679
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4008..4596
/direction=RIGHT
/note="pUC ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
661 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
721 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
781 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt caccaagcac
841 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
901 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
961 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
1021 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
1081 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
1141 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
1201 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
1261 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
1321 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
1381 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
1441 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
1501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
1561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
1621 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
1681 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
1741 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
1801 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
1861 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
1921 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
1981 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2041 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
2101 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
2161 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
2221 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
2281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
2341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
2401 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
2461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
2521 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
2581 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
2641 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
2701 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
2761 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
2821 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
2881 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
2941 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
3001 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
3061 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
3121 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
3181 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
3241 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
3301 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
3361 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
3421 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
3481 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
3541 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
3601 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
3661 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
3721 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
3781 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
3841 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3901 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
3961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
4021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
4081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
4141 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
4201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
4261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
4321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
4381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
4441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
4501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
4681 tctgtggata accgtattac cgccatgcat
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