LOCUS Exported 4711 bp ds-DNA circular SYN 18-10-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4711)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-18 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4711
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 59..362
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 363..566
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 611..1285
/codon_start=1
/product="improved tetrameric variant of DsRed fluorescent
protein"
/note="DsRed2"
/note="mammalian codon-optimized"
/translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
LFL"
CDS 1301..1321
/codon_start=1
/locus_tag="
"
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/note="
"
/protein_id="
"
/translation="PKKKRKV"
CDS 1325..1345
/codon_start=1
/locus_tag="
"
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/note="
"
/protein_id="
"
/translation="PKKKRKV"
CDS 1349..1369
/codon_start=1
/locus_tag="
"
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/note="
"
/protein_id="
"
/translation="PKKKRKV"
polyA_signal 1499..1620
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1627..2082)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2109..2213
/gene="bla"
/note="AmpR promoter"
promoter 2215..2572
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2423..2558
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2607..3401
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3633..3680
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4009..4597
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
61 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
121 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
181 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
241 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
301 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
361 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
421 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
481 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
541 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
601 ggtcgccacc atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg
661 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
721 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
781 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
841 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
901 gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg
961 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca
1021 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
1081 gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
1141 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc
1201 caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac
1261 cgagggccgc caccacctgt tcctgagatc tcgagctgat ccaaaaaaga agagaaaggt
1321 agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag gatccaccgg
1381 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
1441 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
1501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
1561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
1621 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
1681 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
1741 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
1801 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
1861 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
1921 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
1981 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
2041 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
2101 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
2161 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
2221 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
2281 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
2341 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
2401 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
2461 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
2521 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
2581 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
2641 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
2701 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
2761 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
2821 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
2881 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
2941 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
3001 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
3061 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
3121 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
3181 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
3241 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
3301 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
3361 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
3421 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
3481 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
3541 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
3601 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
3661 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
3721 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
3781 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
3841 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
3901 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
3961 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
4021 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
4081 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
4141 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
4201 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
4261 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
4321 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
4381 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
4441 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
4501 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
4561 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
4621 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
4681 ttctgtggat aaccgtatta ccgccatgca t
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