LOCUS Exported 5863 bp ds-DNA circular SYN 21-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5863
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(474..1062)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1233..2093)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2094..2198)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2224..2679)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 2872..3006
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 3023..3180
/label=IgK-IFN
CDS 3210..4862
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 5086..5151
/label=small t intron
/note="simian virus 40 (SV40) small t antigen intron"
CDS 5281..5301
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5726..5860
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 ctcgaggggg ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg
61 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
241 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
301 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
361 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
1021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
1081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
1141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
1201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
1261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
2041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
2101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
2161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
2221 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
2281 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
2341 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
2401 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
2461 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
2521 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
2581 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
2641 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
2701 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
2761 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
2821 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gaacttgttt
2881 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
2941 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
3001 tggatcgatc cccccaccgc ggtggcggcc cacagagggg actttccgag agatctacag
3061 aggggacttt ccgagagcga gcttgggctg caggtcgaga tccgctgaat agagagagga
3121 ccatctcata taaataggcc atacccatgg agaaaggaca ttctaactgc aacctttcgc
3181 aagcttggca ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc
3241 cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga
3301 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca
3361 tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
3421 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
3481 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
3541 aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa
3601 aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta
3661 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
3721 atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa
3781 caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc
3841 cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa
3901 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
3961 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
4021 agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc
4081 tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
4141 aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct
4201 tccatcttcc agggatacga caaggatatg ggctcactga gactacatca gctattctga
4261 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
4321 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat
4381 gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
4441 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
4501 acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc
4561 ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag
4621 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
4681 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
4741 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
4801 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt
4861 aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta ttgtaatatt atatgcaaat
4921 tgatgaatgg taattttgta attgtgggtc actgtactat tttaacgaat aataaaatca
4981 ggtataggta actaaaaagg aattcggggg atctttgtga aggaacctta cttctgtggt
5041 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
5101 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
5161 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
5221 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
5281 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
5341 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
5401 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
5461 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
5521 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
5581 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
5641 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
5701 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
5761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
5821 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gac
//